High impact information on yemalpha

• This cluster of maternally acting genes (yema) maps to 98 F3-10 on chromosome arm 3 R [1].
• Isolation and characterization of a region of the Drosophila genome which contains a cluster of differentially expressed maternal genes (yema gene region) [1].
• We show that the 5' coding sequence is necessary for the early accumulation of yem-alpha RNA in the oocyte and for its localization pattern during oogenesis [2].
• The sequence analysis of the 5696 bp EcoRI fragment containing the yemanuclein-alpha gene, and of 5 overlapping cDNAs, reveals a 3006 nucleotides long open reading frame (ORF) flanked by long untranslated 5' and 3' sequences [3].
• The Drosophila yG 4.5 gene (now called yemanuclein-alpha gene), which maps at 98F, is a member of the yema gene cluster isolated in a search for differentially expressed maternal genes [3].

Biological context of yemalpha

• The yemanuclein-alpha: a new Drosophila DNA binding protein specific for the oocyte nucleus [3].
• Expression in the central nervous system of a subset of the yema maternally acting genes during Drosophila embryogenesis. Post-embryonic expression extends to imaginal discs and spermatocytes [4].

Anatomical context of yemalpha

• We show in the present work that the yemanuclein-alpha is a nuclear protein highly specific for the oocyte nucleus [3].
• The yemanuclein-alpha transcript (formerly yT 4.5) is specifically expressed in the female germ cells at early oogenic stages and displays a graded distribution along the antero-posterior axis of the oocyte [3].

Analytical, diagnostic and therapeutic context of yemalpha

• We demonstrate the DNA binding activity of the yemanuclein-alpha by affinity chromatography experiments [3].
• Using RNA dot-blot analysis and in situ hybridization to tissue sections, we have realized a comprehensive survey of the temporal and spatial expression of the yema transcripts [4].

References