High impact information on Vha16

• Evidence that the 16 kDa proteolipid (subunit c) of the vacuolar H(+)-ATPase and ductin from gap junctions are the same polypeptide in Drosophila and Manduca: molecular cloning of the Vha16k gene from Drosophila [1].
• A pilot study for gene inactivation shows that transposable P-elements can be easily inserted into the Drosophila ductin Vha16 gene [1].
• Previously, these antisera have been shown to recognize, in ovarian follicles and young embryos of Drosophila, the ductin monomer of 16 kDa and a putative dimer of 29 kDa [2].
• To analyze the role of gap-junction mediated cell-cell communication during Drosophila embryogenesis, we used two different polyclonal anti-ductin sera [3].

Analytical, diagnostic and therapeutic context of Vha16

• On immunofluorescence preparations and immunoblots, the anti-ductin sera specifically recognized ovarian as well as embryonic antigens [3].
• Moreover, microinjections into the mid-ventral region of the embryos resulted in specific ventral defects that depended on the concentration of the ductin antibodies [3].
• In order to localize and characterize these proteins in different tissues of Drosophila, we applied indirect immunofluorescence microscopy and immunoblots, using antisera prepared against Drosophila ductin and against Nephrops ductin [2].

References