Disease relevance of NEK3

• Chromosomal region 13q14 is frequently deleted in prostate cancer. nek3, a protein kinase related gene, is located on this region [1].

High impact information on NEK3

• Nek8 activity is not cell cycle regulated, but like Nek3, levels are consistently higher in G(0)-arrested cells [2].
• Novel association of Vav2 and Nek3 modulates signaling through the human prolactin receptor [3].
• Activation of guanosine triphosphatase Rac-1 in Chinese hamster ovary transfectants required both Nek3 and Vav2 and was inhibited by the overexpression of a kinase inactivating Nek3 mutant [3].
• Overexpression of kinase inactive Nek3 in T47D cells led to a 50% increase in apoptosis vs. controls [3].
• However, overexpression of either Nek3 or kinase-inactive Nek3 had no effect on Vav2-potentiated signal transducer and activator of transcription 5-mediated gene expression [3].

Biological context of NEK3

• NEK3 mRNA was detected in all the proliferating cell lines studied, and the amount did not change during the cell cycle [4].
• The human NEK3 gene was assigned to human chromosome 13 by somatic cell hybrids and 13q14.2 by radiation hybrid mapping [4].
• Tpte was mapped by radiation hybrid to a region of mouse chromosome 8 that shows conserved synteny with human 13q14.2-q21 between NEK3 and SGT1 [5].
• In addition we found a variant human NEK3 transcript, which lacks the entire exon 10 due to alternative splicing [1].

Anatomical context of NEK3

• The expression pattern of NEK3 was studied by RT-PCR and a high level of expression was detected in testis, ovary, and brain, with low-level expression being detected in most of the tissues studied [4].

Other interactions of NEK3

• Here, we report the isolation of complementary DNAs encoding Nek2 and Nek3, two novel human protein kinases structurally related to NIMA [6].

Analytical, diagnostic and therapeutic context of NEK3

• We have cloned a cDNA for human NEK3, a novel human gene structurally related to NIMA, by RT-PCR [4].
• Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3) [4].

References