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Gene Review

Nucb2  -  nucleobindin 2

Mus musculus

Synonyms: AI607786, Calnuc, DNA-binding protein NEFA, NEFA, Nefa, ...
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Disease relevance of Nucb2

  • NEFA-green fluorescent protein (GFP) fusion protein expressed in neuroblastoma N1E-115 cells was retained in the cytoplasm and partly secreted into the culture medium [1].

High impact information on Nucb2


Biological context of Nucb2

  • Subjects with the R/G genotype also had lower fasting (448+/-31 micromol/l, p=0.018) and 2-h serum NEFA concentrations (61+/-7 micromol/l, p=0.015) than subjects with the A/A genotype (529+/-9 and 75+/-2 micromol/l, respectively), adjusted for age, sex and percentage of body fat [3].
  • Our aims were to relate cell death to intracellular triglyceride concentration, composition and localisation following incubation of INS1 cells in saturated and unsaturated NEFA in high and low glucose concentrations [4].
  • We investigated possible associations between single nucleotide polymorphisms and percentage of body fat, glucose tolerance (as determined by OGTT), serum NEFA concentrations and whole-body insulin sensitivity (estimated during the OGTT, and for a subgroup of 242 subjects determined by the euglycaemic-hyperinsulinaemic clamp) [3].
  • A QTL for HDL-cholesterol was identified on chromosome 1 and a QTL for NEFA on chromosome 9 [5].

Anatomical context of Nucb2

  • The result was confirmed by the expression of NEFA-green fluorescent protein (GFP) fusion protein in the Golgi in the same cell line [6].

Associations of Nucb2 with chemical compounds

  • Cycloheximide treatment proved NEFA to be a Golgi-resident protein [6].
  • CONCLUSIONS/INTERPRETATION: A novel functional variant in the promoter of the Par6alpha gene is associated with reduced fasting glycaemia, increased glucose tolerance and reduced serum NEFA concentrations [3].
  • Triglyceride content (control cells; 14.5 ng/mug protein) increased up to 10-fold following incubation in NEFA (oleic acid 153.2 ng/mug protein; p<0.05) and triglyceride and phospholipid fractions were both enriched with the specific fatty acid added to the medium (p<0.05) [4].
  • On the other hand, serum total cholesterol, triglycerides, NEFA were observed [7].
  • After 12 wk of intervention, plasma triglyceride, NEFA, and glucose concentrations were significantly higher in the trans10, cis12-CLA group, whereas plasma triglyceride, NEFA, glucose, and insulin concentrations were significantly lower in the cis9, trans11-CLA group, compared with control mice consuming linoleic acid [8].


  1. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. Taniguchi, N., Taniura, H., Niinobe, M., Takayama, C., Tominaga-Yoshino, K., Ogura, A., Yoshikawa, K. J. Biol. Chem. (2000) [Pubmed]
  2. Monocyte chemoattractant protein-1-induced tissue inflammation is critical for the development of renal injury but not type 2 diabetes in obese db/db mice. Chow, F.Y., Nikolic-Paterson, D.J., Ma, F.Y., Ozols, E., Rollins, B.J., Tesch, G.H. Diabetologia (2007) [Pubmed]
  3. A novel functional polymorphism (-336A/G) in the promoter of the partitioning-defective protein-6alpha gene is associated with increased glucose tolerance and lower concentrations of serum non-esterified fatty acids. Weyrich, P., Lammers, R., Fritsche, A., Machicao, F., Häring, H.U., Stefan, N. Diabetologia (2005) [Pubmed]
  4. Adverse physicochemical properties of tripalmitin in beta cells lead to morphological changes and lipotoxicity in vitro. Moffitt, J.H., Fielding, B.A., Evershed, R., Berstan, R., Currie, J.M., Clark, A. Diabetologia (2005) [Pubmed]
  5. Quantitative trait loci that regulate plasma lipid concentration in hereditary obese KK and KK-Ay mice. Suto, J., Matsuura, S., Yamanaka, H., Sekikawa, K. Biochim. Biophys. Acta (1999) [Pubmed]
  6. Golgi retention of human protein NEFA is mediated by its N-terminal Leu/Ile-rich region. Nesselhut, J., Jurgan, U., Onken, E., Götz, H., Barnikol, H.U., Hirschfeld, G., Barnikol-Watanabe, S., Hilschmann, N. FEBS Lett. (2001) [Pubmed]
  7. Effect of Garcinia cambogia extract on serum leptin and insulin in mice. Hayamizu, K., Hirakawa, H., Oikawa, D., Nakanishi, T., Takagi, T., Tachibana, T., Furuse, M. Fitoterapia (2003) [Pubmed]
  8. Divergent mechanisms of cis9, trans11-and trans10, cis12-conjugated linoleic acid affecting insulin resistance and inflammation in apolipoprotein E knockout mice: a proteomics approach. de Roos, B., Rucklidge, G., Reid, M., Ross, K., Duncan, G., Navarro, M.A., Arbones-Mainar, J.M., Guzman-Garcia, M.A., Osada, J., Browne, J., Loscher, C.E., Roche, H.M. FASEB J. (2005) [Pubmed]
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