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Gene Review

Nucb2  -  nucleobindin 2

Rattus norvegicus

Synonyms: DNA-binding protein NEFA, Nefa, Nucleobindin-2, Prepronesfatin, p54
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High impact information on Nucb2

  • In nuclear pores p62 forms a tight complex with at least two other proteins, p58 and p54, which can be extracted from isolated rat liver nuclei (Finlay, D. R., E. Meier, P. Bradley, J. Horecka, and D. J. Forbes. 1991. J. Cell Biol. 114:169-183) [1].
  • Although distinguishable in their substrate specificity, these protein kinases together with the p54 MAP-2 kinase share a major common specificity determinant reflected in the SKAIPS peptide: the requirement for a proline residue immediately carboxyl-terminal to the site of Ser/Thr phosphorylation [2].
  • Analysis of the Sephacryl fractions by SDS-PAGE indicates that a major Coomassie blue-stained band with an apparent Mr = 54,000 (p54), co-elutes with the peak of kinase activity [3].
  • Glutamate increased the phosphorylation of p54-c-jun N-terminal kinase (JNK) concomitantly with activation of the endogenous antioxidant defense system [4].
  • Ethanol also activated both p42/44 MAPK and p46/p54 JNK [5].

Anatomical context of Nucb2

  • By immunofluorescence, p54/NEFA essentially colocalized with the medial Golgi marker mannosidase II, and did not overlap with the cis-Golgi marker p58, nor with the trans-Golgi network (TGN) marker TGN38 [6].
  • The stress-activated protein kinases p46 JNK, p54 JNK, and p38 SAPK are activated by hepatocyte isolation and precede changes in gene 33 mRNA content [7].
  • It was recently demonstrated that activation of the mechanically-sensitive kinase p54 jun-N-terminal-kinase (JNK), is a quantitative marker of mechanical stimulation in skeletal muscle [8].

Associations of Nucb2 with chemical compounds

  • The calcium-binding protein p54/NEFA is a novel luminal resident of medial Golgi cisternae that traffics independently of mannosidase II [6].
  • On day 7 of DEX stimulation, the presence of valinomycin resulted in low p54/52src [9].

Analytical, diagnostic and therapeutic context of Nucb2

  • FR167653 inhibited the activation of p38 MAPK both 30 and 60 min after reperfusion, but it did not affect the activation of p46/p54 JNKs [10].
  • Activation of p38 MAPK and JNKs was assessed at the different times of the experimental setting by standard Western blotting techniques using a dual phospho p38MAPK and phospho JNKs (p46/p54) antibodies [11].


  1. Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: binding of nucleoporin p54 to the rod domain of p62. Buss, F., Stewart, M. J. Cell Biol. (1995) [Pubmed]
  2. An array of insulin-activated, proline-directed serine/threonine protein kinases phosphorylate the p70 S6 kinase. Mukhopadhyay, N.K., Price, D.J., Kyriakis, J.M., Pelech, S., Sanghera, J., Avruch, J. J. Biol. Chem. (1992) [Pubmed]
  3. Purification of a tyrosine-specific protein kinase from Rous sarcoma virus-induced rat tumor. Blithe, D.L., Richert, N.D., Pastan, I.H. J. Biol. Chem. (1982) [Pubmed]
  4. Tournefolic acid B methyl ester attenuates glutamate-induced toxicity by blockade of ROS accumulation and abrogating the activation of caspases and JNK in rat cortical neurons. Chi, C.W., Wang, C.N., Lin, Y.L., Chen, C.F., Shiao, Y.J. J. Neurochem. (2005) [Pubmed]
  5. Temporal activation of p42/44 mitogen-activated protein kinase and c-Jun N-terminal kinase by acetaldehyde in rat hepatocytes and its loss after chronic ethanol exposure. Lee, Y.J., Aroor, A.R., Shukla, S.D. J. Pharmacol. Exp. Ther. (2002) [Pubmed]
  6. The calcium-binding protein p54/NEFA is a novel luminal resident of medial Golgi cisternae that traffics independently of mannosidase II. Morel-Huaux, V.M., Pypaert, M., Wouters, S., Tartakoff, A.M., Jurgan, U., Gevaert, K., Courtoy, P.J. Eur. J. Cell Biol. (2002) [Pubmed]
  7. Activation of stress-activated protein kinases by hepatocyte isolation induces gene 33 expression. Varley, C.L., Armitage, S., Dickson, A.J. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  8. Skeletal muscle is sensitive to the tension-time integral but not to the rate of change of tension, as assessed by mechanically induced signaling. Martineau, L.C., Gardiner, P.F. Journal of biomechanics. (2002) [Pubmed]
  9. Src protein and tyrosine-phosphorylated protein profiles in marrow stroma during osteogenic stimulation. Klein, B.Y., Levitzki, R., Ben-Bassat, H. J. Cell. Biochem. (1998) [Pubmed]
  10. Effects of a p38 mitogen-activated protein kinase inhibitor as an additive to university of wisconsin solution on reperfusion injury in liver transplantation. Yoshinari, D., Takeyoshi, I., Kobayashi, M., Koyama, T., Iijima, K., Ohwada, S., Matsumoto, K., Morishita, Y. Transplantation (2001) [Pubmed]
  11. Thyroid hormone and cardioprotection: study of p38 MAPK and JNKs during ischaemia and at reperfusion in isolated rat heart. Pantos, C., Malliopoulou, V., Paizis, I., Moraitis, P., Mourouzis, I., Tzeis, S., Karamanoli, E., Cokkinos, D.D., Carageorgiou, H., Varonos, D., Cokkinos, D.V. Mol. Cell. Biochem. (2003) [Pubmed]
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