High impact information on LOC548245

• One of the recently identified jasmonate-induced proteins, designated JIP60, in barley is a ribosome-inactivating protein that cleaves polysomes of both animal and plant origin into their ribosomal subunits [1].
• The rise in jasmonates is accompanied by the expression of genes coding for an enzyme of JA biosynthesis (allene oxide synthase) and of a jasmonate-induced protein (JIP23) [2].
• Nucleotide sequence of a cDNA from Atriplex canescens (Pursh.) Nutt. A homolog of a jasmonate-induced protein from barley [3].
• The N-terminal region of a 60 kDa, jasmonate-induced protein of barley leaves (JIP60) is shown to be homologous to the catalytic domains of plant ribosome-inactivating proteins (RIP) [4].
• JIP60 derivatives do not significantly inhibit in vitro protein synthesis on wheat germ ribosomes [4].

Biological context of LOC548245

• Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of chloroplasts [5].

Anatomical context of LOC548245

• Based on quantitative measurements and fluorescence microscopy, jasmonate-induced accumulation of ferulic acid and phenolic polymers in the cell wall were detected and the possibility of cell wall strengthening mediated through phenolic crosslinks is discussed [6].

Associations of LOC548245 with chemical compounds

• The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare) [7].
• Also a rise of endogenous jasmonic acid resulting from sorbitol stress did not induce LOX2:Hv:1, suggesting a separate signalling pathway compared with other jasmonate-induced proteins of barley [8].
• These data revealed jasmonate-induced activation of the hydroperoxide lyase and reductase branch within the lipoxygenase pathway and suggest differential activity of the three 13-lipoxygenases under different stress conditions [5].
• A gene sequence in barley that earlier was characterized as a jasmonate-induced O-methyltransferase (MT) (EC 2.1.1.6, GenBank accession U54767) was here found to be absent in some barley cultivars and breeding lines that all lacked gramine [9].
• Jasmonate-induced changes in flavonoid metabolism in barley (Hordeum vulgare) leaves [10].

Analytical, diagnostic and therapeutic context of LOC548245

• Western blotting of leaf extracts and in vitro reconstitution experiments indicate that JIP60 is synthesized as a precursor which is processed in vivo [4].
• In situ hybridization revealed specific accumulation of JIP-23 mRNA in companion cells of the phloem in the nodule plate of the scutellum [11].

References