Disease relevance of TSKS

• The expression of the TSKS gene was very low in two human embryonal carcinoma cell lines, 2102Ep and NTERA-2 [1].
• TSKS expression was very low or undetectable in seminoma, teratocarcinoma, embryonal, and Leydig cell tumors, while high expression was observed in testicular tissue adjacent to tumors which contain premalignant carcinoma in situ [1].

High impact information on TSKS

• TSSK 2 expressed in yeast and bacteria was able to autophosphorylate and also phosphorylated recombinant TSKS in vitro [2].
• In contrast, TSKS was only found in the testis [2].
• These observations suggest a role of TSKS in testicular physiology, most probably in the process of spermatogenesis or spermiogenesis [1].
• This gene (TSKS, for testis-specific kinase substrate) is composed of 11 exons and 10 intervening introns and is likely the human homolog of the mouse testis-specific serine kinase substrate gene [1].
• Mean skin temperature (Tsk) of the control increased gradually after the onset of sweating, while the Tsks at 25%O(2) and 30%O(2) maintained a constant level during sweating [3].

Other interactions of TSKS

• Human TSSK 2 and TSKS interacted in a yeast two-hybrid system and also co-immunoprecipitated after in vitro translation [2].

Analytical, diagnostic and therapeutic context of TSKS

• TSKS had an ORF of 592 amino acids and was also expressed exclusively in the testis as demonstrated by northern and dot blot analyses; however, lower levels of expression in other tissues were detected using real-time PCR [2].

References