High impact information on Impg1

• Photoreceptors project from the outer retinal surface into a specialized glycocalyx, the interphotoreceptor matrix (IPM), which contains hyaluronan (HA) and two novel proteoglycans, Spacr and Spacrcan [1].
• Multitissue Northern blot analysis and in situ hybridization studies indicate that SPACR expression is restricted to retinal photoreceptors [2].
• Alignment comparisons of the deduced amino acid sequence of mouse and human SPACR show 64% homology [2].
• Mouse SPACR cDNA comprises 3675 bp containing an open reading frame coding for 742 amino acids [2].
• Mouse SPACR cDNA was cloned by screening a mouse retina cDNA library using a PCR probe derived from human SPACR cDNA [2].

Associations of Impg1 with chemical compounds

• Recent biochemical studies of human and mouse SPACR protein indicate that this novel interphotoreceptor matrix molecule is a glycoprotein in human and a proteoglycan in the mouse [2].

Analytical, diagnostic and therapeutic context of Impg1

• The SPACR core protein was identified with Western blotting following SDS-PAGE with a SPACR C-terminal peptide polyclonal antibody and a chondroitin-6-sulfate Deltadisaccharide monoclonal antibody [2].

References