High impact information on ST8

• The MRSA strains that were sequence type 8 (ST8), staphylococcal cassette chromosome mec (SCCmec) type IV, and Panton-Valentine leukocidin-positive clustered separately from those that were ST5 and SCCmec type II [1].
• The overwhelming majority of the isolates-187 of 202, or 93%-belonged to one of three internationally spread epidemic clones which were identified on the basis of their multilocus sequence type (ST) as E-MRSA-16 (ST36), the New York clone V (ST8), and the New York/Japan clone (ST5; SCCmec II) and its single- and double-locus variants [2].
• The detection limit observed for ST8 purified from K562 cells was approximately 2 pg by dot-blot analysis [3].
• This paper reports the development of a synthetic probe composed of oxidized colominic acid coupled to biotinyl-L-lysine hydrazide to detect and quantify ST8 with putative "initiase" activity and its use in three solid-phase applications [3].
• PCRs for cataloguing the staphylococcal cassette chromosome (SCCmec) type were negative for two of these, suggesting the existence of a new methicillin-resistance gene complex in the ST8 genetic background [4].

Biological context of ST8

• The most common PVL-MRSA genotypes were sequence type ST8, ST22, ST30, ST59 and ST80 [5].
• The results showed that serogroup C meningococci were predominant, and that a dramatic increase in the circulation of strains with decreased susceptibility to penicillin was associated mainly with a prevalent phenotype C:2b:P1.5,2, which belongs to the hyper-virulent ST8/A4 cluster [6].

Other interactions of ST8

• The E-modulus of Novalyse ST8, ST14 and ST20 mixed with contrast agent was 18, 23 and 33kPa, respectively [7].

Analytical, diagnostic and therapeutic context of ST8

• In Western blots the probe bound and specifically recognized a protein band corresponding to ST8 [3].
• Analysis of 3'-azido-3'deoxythymidine-treated cells by all three methods showed a reduction in ST8 compared to control cells; treated cells had 73% of control levels by ELISA [3].
• However, six cases, all with good outcomes, were caused by C:2b:(P1.2,P1.5) isolates of the same pulsed-field gel electrophoresis type belonging to ST8 complex/Cluster A4 [8].

References