High impact information on UBE2E2

• Furthermore, these results raise the possibility that the ISG15 conjugation pathway might use UbcH8-competent Ub ligases in vivo [1].
• Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein [2].
• Moreover, siRNA-mediated down-regulation of the major E2 for ISG15 (UbcH8), which blocked the formation of ISG15 protein conjugates, also increased the levels of polyubiquitinated proteins [3].
• We have previously cloned cDNAs encoding the N-terminally extended class III human ubiquitin-conjugating enzymes (E2s), UBE2E2 and UBE2E3, the biological functions of which are not known [4].
• In this study, we performed yeast two-hybrid screening for protein(s) interacting with UBE2E2, and two RING-finger proteins, ARA54 and RNF8, were identified [4].

Biological context of UBE2E2

• The UBE2E2 gene was assigned to human chromosome 3p24.2 by fluorescence in situ hybridization [5].
• cDNA cloning, characterization, and chromosome mapping of UBE2E2 encoding a human ubiquitin-conjugating E2 enzyme [5].
• From a cDNA library of human lymph nodes, a cDNA encoding the ubiquitin-conjugating enzyme UbcH8 was identified [6].

Associations of UBE2E2 with chemical compounds

• The Spen Homolog Msx2-Interacting Nuclear Target Protein Interacts with the E2 Ubiquitin-Conjugating Enzyme UbcH8 [6].

Analytical, diagnostic and therapeutic context of UBE2E2

• The interaction between MINT and UbcH8 in mammalian cells was further proved by a series of biochemical assays including the mammalian two-hybrid assay, GST pull-down assay, and co-immunoprecipitation assay [6].

References