High impact information on PFL

• We have cloned the wild-type PFL gene by T-DNA tagging, and demonstrate that it complements the mutant phenotype [1].
• PFL codes for ribosomal protein S18, based on the high homology with rat S18 and on purification of S18-equivalent peptides from plant ribosomes. pfl represents the first mutation in eukaryotic S18 proteins or their S13 prokaryotic counterparts, involved in translation initiation [1].
• An S18 ribosomal protein gene copy at the Arabidopsis PFL locus affects plant development by its specific expression in meristems [1].
• Sequence analysis of a 24-kb contiguous genomic region at the Arabidopsis thaliana PFL locus on chromosome 1 [2].
• The cosmid is located on chromosome 1 at the PFL locus (position 30 cM) [2].

Biological context of PFL

• Genomic and BAC Southern analyses indicated that there is only one copy of PFL in the papaya genome [3].
• These data suggest that PFL may play a similar role as LFY in flower development and has limited effect on sex differentiation in papaya [3].

Analytical, diagnostic and therapeutic context of PFL

• Quantitative PCR analyses revealed that PFL was expressed in flower buds of all three sex types - male, female, and hermaphrodite with marginal difference between hermaphrodite and unisexual flowers [3].
• In situ hybridization experiments demonstrated that PFL is expressed at a relatively low level in leaf primordia, but it is expressed at a high level in the floral meristem [3].

References