High impact information on FKF1

• FKF1 mRNA levels oscillate with a circadian rhythm, and deletion of FKF1 alters the waveform of rhythmic expression of two clock-controlled genes, implicating FKF1 in modulating the Arabidopsis circadian clock [1].
• We positionally cloned FKF1, which encodes a novel protein with a PAS domain similar to the flavin-binding region of certain photoreceptors, an F box characteristic of proteins that direct ubiquitin-mediated degradation, and six kelch repeats predicted to fold into a beta propeller [1].
• Studies of the plant phototropins have identified 11 flavin-contacting residues that are also conserved in the LOV domains of WC-1, VVD, and FKF1 [2].
• Furthermore, we show that the Neurosporastrains containing a chimeric WC-1 protein with the LOV domain from FKF1 or phot1 can also sense light, suggesting that FKF1 and its related proteins are light sensors in Arabidopsis [2].
• Flavin-binding, Kelch repeat, F-box (FKF1) protein is a photoreceptor to regulate flowering of Arabidopsis [3].

Regulatory relationships of FKF1

• Thus, FKF1 controls daily CO expression in part by degrading CDF1, a repressor of CO transcription [4].

Other interactions of FKF1

• LKP2 interacted not only with itself but also with other members of the family, LKP1 and FKF1 [5].
• We demonstrate here that FKF1 controls the stability of a Dof transcription factor, CYCLING DOF FACTOR 1 (CDF1) [4].
• FKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis [4].

Analytical, diagnostic and therapeutic context of FKF1

• In the present study, a LOV-containing polypeptide of FKF1 was prepared by an overexpression system, and the quaternary structure of it was studied by size exclusion chromatography and small-angle X-ray scattering [3].

References