High impact information on GIT1

• Glycerophosphocholine-dependent growth requires Gde1p (YPL110c) and Git1p in Saccharomyces cerevisiae [1].
• Furthermore, glycerophospho[3H]choline accumulation requires the transporter encoded by GIT1, a known glycerophosphoinositol transporter [1].
• A 300-base pair region of the GIT1 promoter containing potential Pho4p binding sites was shown to be required for full GIT1 expression [2].
• In contrast, Ino2p and Ino4p are required for full GIT1 expression when inositol is limiting, with or without phosphate limitation, but not when only phosphate is limiting [2].
• GIT1 transcript levels and Git1p activity are greater in cells starved for phosphate, with or without inositol limitation, than in cells only limited for inositol [2].

Biological context of GIT1

• Unlike P(i), high levels of GroPCho and GroPIns do not repress GIT1 promoter-driven reporter gene activity [3].
• Furthermore, the kinetics of GIT1 transcript accumulation and Git1p activity upon transfer of cells to phosphate starvation media are different from those obtained upon transfer of cells to inositol-free media [2].
• Mutations that inhibit endocytosis and vacuolar proteolysis inhibit Git1p degradation, indicating that Git1p downregulation involves internalization and subsequent degradation in the vacuole [3].

Anatomical context of GIT1

• GroPIns and GroPCho are transported across the Saccharomyces cerevisiae plasma membrane into the cell via the transporter encoded by GIT1 [3].

Associations of GIT1 with chemical compounds

• Growth on glycerophosphocholine as the sole phosphate source requires YPL110c and the Git1p permease [1].

Other interactions of GIT1

• Deletion of PHO4 abolished GIT1 transcription in a wild-type strain [4].

References