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Gene Review

MTF2  -  Mtf2p

Saccharomyces cerevisiae S288c

Synonyms: D2705, Mitochondrial transcription factor 2, NAM1, Protein NAM1, YDL044C
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High impact information on MTF2

  • This connection is mediated in large part by its interactions with the matrix protein Nam1p and, based on genetic phenotypes, the mitochondrial membrane protein Sls1p [1].
  • Here we demonstrate by specific labeling of mitochondrial gene products in vivo that Nam1p and Sls1p indeed work together in a pathway that is required globally for efficient mitochondrial translation [1].
  • In addition, mutations in the amino-terminal domain result specifically in decreased steady-state levels of mature mitochondrial CYTB and COXI transcripts, which is a primary defect observed in NAM1 null mutant yeast strains [2].
  • Phenotypic analysis of deletion and point mutations, in conjunction with a yeast two-hybrid assay, revealed that Nam1p, a protein involved in RNA processing and translation in mitochondria, binds specifically to this domain [2].
  • Change of serine309 into proline causes temperature sensitivity of the nuclear NAM1/MTF2 gene product for yeast mitochondria [3].

Biological context of MTF2

  • Comparison of the 5' upstream regions of MTF2 and a previously characterized mitochondrial transcription factor (MTF1) revealed common sequence motifs which may be important for coordinated regulation of gene expression [4].
  • A fusion plasmid enabling bacterial over-expression of the corresponding protein-A-NAM1 cognate was constructed and subsequently employed as an antigen to raise polyclonal antibodies [5].
  • The significance of this interaction in vivo was demonstrated by the fact that the temperature-sensitive phenotype of a deletion mutation (rpo41Delta2), which impinges on this amino-terminal domain, is suppressed by overproducing Nam1p [2].
  • These nuclear genes show no sequence homology to each other and are localized on different chromosomes: NAM1 on chromosome IV, NAM7 on chromosome XIII and NAM8 on chromosome VIII [6].

Anatomical context of MTF2

  • These antibodies specifically recognise a 50-kDa protein which purifies along with the mitochondria and corresponds to NAM1p [5].

Regulatory relationships of MTF2

  • The NAM1 nuclear gene was shown to control the stability and/or processing of mitochondrial transcripts of the cytochrome b, cytochrome oxidase subunit I and ATP synthase subunit VI genes [Groudinsky O., Bousquet I., Wallis M. G., Slonimski, P. P. & Dujardin G. (1993) Mol. Gen. Genet. 240, 419-427] [5].

Other interactions of MTF2

  • How Pet309p may function in concert with other gene products necessary for COX1 RNA translation or accumulation, such as Mss51p or Nam1p, respectively, is discussed [7].
  • This was supported by the observation that SLS1 overexpression rescued the petite phenotype of a NAM1 null mutation [8].

Analytical, diagnostic and therapeutic context of MTF2

  • To determine which step in mitochondrial RNA metabolism is controlled in vivo by the NAM1 gene, mitochondrial transcripts of seven transcription units from strains carrying an inactive nam1::URA3 gene disruption in various mitochondrial genetic backgrounds were analysed by Northern blot hybridisations [9].
  • Sequence analysis of the NAM1 gene shows that it encodes a protein of 440 amino acids with a typical presequence that would target the protein to the mitochondrial matrix [6].


  1. Multiple interactions involving the amino-terminal domain of yeast mtRNA polymerase determine the efficiency of mitochondrial protein synthesis. Rodeheffer, M.S., Shadel, G.S. J. Biol. Chem. (2003) [Pubmed]
  2. Nam1p, a protein involved in RNA processing and translation, is coupled to transcription through an interaction with yeast mitochondrial RNA polymerase. Rodeheffer, M.S., Boone, B.E., Bryan, A.C., Shadel, G.S. J. Biol. Chem. (2001) [Pubmed]
  3. Change of serine309 into proline causes temperature sensitivity of the nuclear NAM1/MTF2 gene product for yeast mitochondria. Lisowsky, T., Riemen, G., Michaelis, G. Nucleic Acids Res. (1990) [Pubmed]
  4. Molecular analysis of the mitochondrial transcription factor mtf2 of Saccharomyces cerevisiae. Lisowsky, T. Mol. Gen. Genet. (1990) [Pubmed]
  5. The NAM1 protein (NAM1p), which is selectively required for cox1, cytb and atp6 transcript processing/stabilisation, is located in the yeast mitochondrial matrix. Wallis, M.G., Groudinsky, O., Slonimski, P.P., Dujardin, G. Eur. J. Biochem. (1994) [Pubmed]
  6. Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Asher, E.B., Groudinsky, O., Dujardin, G., Altamura, N., Kermorgant, M., Slonimski, P.P. Mol. Gen. Genet. (1989) [Pubmed]
  7. The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane. Manthey, G.M., Przybyla-Zawislak, B.D., McEwen, J.E. Eur. J. Biochem. (1998) [Pubmed]
  8. Sls1p is a membrane-bound regulator of transcription-coupled processes involved in Saccharomyces cerevisiae mitochondrial gene expression. Bryan, A.C., Rodeheffer, M.S., Wearn, C.M., Shadel, G.S. Genetics (2002) [Pubmed]
  9. The NAM1/MTF2 nuclear gene product is selectively required for the stability and/or processing of mitochondrial transcripts of the atp6 and of the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae. Groudinsky, O., Bousquet, I., Wallis, M.G., Slonimski, P.P., Dujardin, G. Mol. Gen. Genet. (1993) [Pubmed]
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