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Gene Review

TEF2  -  translation elongation factor EF-1 alpha

Saccharomyces cerevisiae S288c

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Disease relevance of TEF2


High impact information on TEF2

  • The promoters of the Ashbya gossypii TEF gene and the S. cerevisiae TEF1 and TEF2 genes, however, are resistant to transcriptional silencing by the HM silencers in yeast [2].
  • In fact, a 149-bp segment consisting essentially of only three tandem Rap1-binding sites from the UASrpg of yeast TEF2 exhibits silencer-blocking activity [2].
  • Complex 2, formed on TEF2 or RP51A probes at higher protein concentrations, corresponded to an extended footprint of 35-40 bp [3].
  • Nucleotide sequence data from restriction fragments isolated from the 5' and 3' ends of TEF-2 and -3 confirmed the presence of a second intervening sequence in these genes [4].
  • A Bni1p-binding protein was affinity purified from the yeast cytosol fraction and was identified to be Tef1p/Tef2p, translation elongation factor 1alpha (EF1alpha) [5].

Biological context of TEF2

  • Nine dominant mutant alleles of TEF2 that cause increased suppression of frameshift mutations also suppressed the lethality of tef5::TRP1 [6].
  • The nucleotide sequence of the cDNA showed significant homology to a region of the Saccharomyces cerevisiae genes for EF-1 alpha (TEF1 and TEF2) [7].
  • A series of Saccharomyces cerevisiae strains containing mutant alleles of the TEF2 gene encoding EF-1alpha have phenotypes consistent with effects on cellular processes related to translation [8].
  • The first plasmid is a centromeric plasmid expressing under the control of a TEF2 promoter the S65T mutant form of Gfp [9].
  • We have generated yeast haploid strains containing either TEF1 or TEF2 interrupted by insertion of a large piece of foreign DNA [10].

Associations of TEF2 with chemical compounds


Regulatory relationships of TEF2

  • TEF2 alone is sufficient to promote growth of the cells as shown with a strain deleted for TEF1 [12].
  • The lethality of a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha [6].

Other interactions of TEF2

  • However, we were unable to detect specific interactions with CTTCC sequence elements found in front of the translational component genes TEF1, TEF2, and CRY1 [13].
  • RAP1 binding to these sites, however, is significantly weaker than that to sites in TEF2 and HMRE [14].
  • Constitutive transcription was not affected, as determined in ADH1 and TEF2 [15].

Analytical, diagnostic and therapeutic context of TEF2

  • Fractionation of yeast extracts on heparin-agarose revealed the presence of a DNA footprinting activity that interacted specifically with the 5'-upstream region of TEF1 and TEF2 genes coding for the protein synthesis elongation factor EF-1 alpha, and of the ribosomal protein gene RP51A [3].
  • Sequence analysis of the two clones showed that regions of DNA flanking the coding regions of the two genes were not homologous, verifying the presence of two genes, called TEF1 and TEF2, for EF-1 alpha in C. albicans [16].


  1. PCR-based gene targeting in the filamentous fungus Ashbya gossypii. Wendland, J., Ayad-Durieux, Y., Knechtle, P., Rebischung, C., Philippsen, P. Gene (2000) [Pubmed]
  2. UASrpg can function as a heterochromatin boundary element in yeast. Bi, X., Broach, J.R. Genes Dev. (1999) [Pubmed]
  3. A general upstream binding factor for genes of the yeast translational apparatus. Huet, J., Cottrelle, P., Cool, M., Vignais, M.L., Thiele, D., Marck, C., Buhler, J.M., Sentenac, A., Fromageot, P. EMBO J. (1985) [Pubmed]
  4. Expression of three genes for elongation factor 1 alpha during morphogenesis of Mucor racemosus. Linz, J.E., Sypherd, P.S. Mol. Cell. Biol. (1987) [Pubmed]
  5. Interaction of Rho1p target Bni1p with F-actin-binding elongation factor 1alpha: implication in Rho1p-regulated reorganization of the actin cytoskeleton in Saccharomyces cerevisiae. Umikawa, M., Tanaka, K., Kamei, T., Shimizu, K., Imamura, H., Sasaki, T., Takai, Y. Oncogene (1998) [Pubmed]
  6. Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta. Kinzy, T.G., Woolford, J.L. Genetics (1995) [Pubmed]
  7. Three genes for the elongation factor EF-1 alpha in Mucor racemosus. Linz, J.E., Katayama, C., Sypherd, P.S. Mol. Cell. Biol. (1986) [Pubmed]
  8. Translational misreading: mutations in translation elongation factor 1alpha differentially affect programmed ribosomal frameshifting and drug sensitivity. Dinman, J.D., Kinzy, T.G. RNA (1997) [Pubmed]
  9. Single-cell variability in growing Saccharomyces cerevisiae cell populations measured with automated flow cytometry. Kacmar, J., Zamamiri, A., Carlson, R., Abu-Absi, N.R., Srienc, F. J. Biotechnol. (2004) [Pubmed]
  10. Either one of the two yeast EF-1 alpha genes is required for cell viability. Cottrelle, P., Cool, M., Thuriaux, P., Price, V.L., Thiele, D., Buhler, J.M., Fromageot, P. Curr. Genet. (1985) [Pubmed]
  11. Mutations in elongation factor EF-1 alpha affect the frequency of frameshifting and amino acid misincorporation in Saccharomyces cerevisiae. Sandbaken, M.G., Culbertson, M.R. Genetics (1988) [Pubmed]
  12. Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae. Schirmaier, F., Philippsen, P. EMBO J. (1984) [Pubmed]
  13. Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae. Huie, M.A., Scott, E.W., Drazinic, C.M., Lopez, M.C., Hornstra, I.K., Yang, T.P., Baker, H.V. Mol. Cell. Biol. (1992) [Pubmed]
  14. Participation of RAP1 protein in expression of the Saccharomyces cerevisiae arginase (CAR1) gene. Kovari, L.Z., Kovari, I., Cooper, T.G. J. Bacteriol. (1993) [Pubmed]
  15. The yeast HRS1 gene is involved in positive and negative regulation of transcription and shows genetic characteristics similar to SIN4 and GAL11. Piruat, J.I., Chávez, S., Aguilera, A. Genetics (1997) [Pubmed]
  16. Sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast Candida albicans. Sundstrom, P., Smith, D., Sypherd, P.S. J. Bacteriol. (1990) [Pubmed]
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