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Gene Review

RPS14A  -  ribosomal 40S subunit protein S14A

Saccharomyces cerevisiae S288c

Synonyms: 40S ribosomal protein S14-A, CRY1, RP59A, RPL59, YCR031C, ...
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Disease relevance of RPS14A


High impact information on RPS14A

  • Extra copies of the cry1 or RPL16 genes encoding ribosomal proteins rp59 or L16 were introduced into yeast by transformation [3].
  • Several mutations within the CRY1 C terminus lead to reduced phosphorylation by phytochrome preparations in vitro [4].
  • Yeast two-hybrid interaction studies using expressed C-terminal fragments of CRY1 and phytochrome A from Arabidopsis confirm a direct physical interaction between both photoreceptors [4].
  • Mutations in cyh2 or cry1, producing resistance to cycloheximide and crytopleurine due to mutant ribosomal proteins, do not produce a mak- phenotype [5].
  • Deletion or inactivation of CRY1 leads to 5- to 10-fold-increased levels of CRY2 mRNA [6].

Biological context of RPS14A

  • Ribosomal protein 59 is an essential protein; upon sporulation of a diploid doubly heterozygous for cry1-delta 2::TRP1 cry2-delta 1::LEU2 null alleles, no spore clones containing both null alleles were recovered [1].
  • The DNA sequence of the CRY2 gene contains an open reading frame encoding ribosomal protein 59 that differs at five residues from rp59 encoded by the CRY1 gene [1].
  • (2) Haploids containing the cry1-delta 2::TRP1 null allele have a deficit of 40S ribosomal subunits, but cry2-delta 1::LEU2 strains have wild-type amounts of 40S ribosomal subunits [1].
  • We report the sequence of a 7.5 kb region lying between the CRY1 and MAT loci of chromosome III from Saccharomyces cerevisiae [7].
  • We found that the HOMOL1 and RPG consensus sequences are not necessary for the heat shock response of CRY1 [2].

Anatomical context of RPS14A

  • Excess cry1 or RPL16 mRNA accumulated in polyribosomes in these cells and was translated at wild-type rates into rp59 or L16 proteins [3].

Associations of RPS14A with chemical compounds

  • Reciprocal expression of RP59 and CRY1 in heterologous hosts demonstrates that glucose upshift occurs in S.cerevisiae but not K.lactis [8].
  • In heterozygous (+/cry1) diploids both the sensitive and the resistant genes are expressed as shown by studies of the action of cryptopleurine on polyphenylalanine-synthesizing systems derived from each parental sensitive and resistant haploid strain and heterozygous diploid strains [9].

Other interactions of RPS14A


Analytical, diagnostic and therapeutic context of RPS14A


  1. Molecular genetics of cryptopleurine resistance in Saccharomyces cerevisiae: expression of a ribosomal protein gene family. Paulovich, A.G., Thompson, J.R., Larkin, J.C., Li, Z., Woolford, J.L. Genetics (1993) [Pubmed]
  2. Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene. Larkin, J.C., Thompson, J.R., Woolford, J.L. Mol. Cell. Biol. (1987) [Pubmed]
  3. Ribosomal protein synthesis is not regulated at the translational level in Saccharomyces cerevisiae: balanced accumulation of ribosomal proteins L16 and rp59 is mediated by turnover of excess protein. Tsay, Y.F., Thompson, J.R., Rotenberg, M.O., Larkin, J.C., Woolford, J.L. Genes Dev. (1988) [Pubmed]
  4. The CRY1 blue light photoreceptor of Arabidopsis interacts with phytochrome A in vitro. Ahmad, M., Jarillo, J.A., Smirnova, O., Cashmore, A.R. Mol. Cell (1998) [Pubmed]
  5. Ribosomal protein L3 is involved in replication or maintenance of the killer double-stranded RNA genome of Saccharomyces cerevisiae. Wickner, R.B., Ridley, S.P., Fried, H.M., Ball, S.G. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  6. Feedback inhibition of the yeast ribosomal protein gene CRY2 is mediated by the nucleotide sequence and secondary structure of CRY2 pre-mRNA. Li, Z., Paulovich, A.G., Woolford, J.L. Mol. Cell. Biol. (1995) [Pubmed]
  7. The complete sequence of a 7.5 kb region of chromosome III from Saccharomyces cerevisiae that lies between CRY1 and MAT. Wicksteed, B.L., Roberts, A.B., Sagliocco, F.A., Brown, A.J. Yeast (1991) [Pubmed]
  8. Altered response to growth rate changes in Kluyveromyces lactis versus Saccharomyces cerevisiae as demonstrated by heterologous expression of ribosomal protein 59 (CRY1). Larson, G.P., Rossi, J.J. Nucleic Acids Res. (1991) [Pubmed]
  9. Genetics and biochemistry of cryptopleurine resistance in the yeast Saccharomyces cerevisiae. Sánchez, L., Vásquez, D., Jiménez, A. Mol. Gen. Genet. (1977) [Pubmed]
  10. Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae. Maddock, J.R., Roy, J., Woolford, J.L. Nucleic Acids Res. (1996) [Pubmed]
  11. Analysis of interchromosomal mitotic recombination. McGill, C.B., Shafer, B.K., Higgins, D.R., Strathern, J.N. Curr. Genet. (1990) [Pubmed]
  12. The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames. Bolle, P.A., Gilliquet, V., Berben, G., Dumont, J., Hilger, F. Yeast (1992) [Pubmed]
  13. Recombination initiated by double-strand breaks. McGill, C.B., Shafer, B.K., Derr, L.K., Strathern, J.N. Curr. Genet. (1993) [Pubmed]
  14. The complete sequence of the unit YCR59, situated between CRY1 and MAT, reveals two long open reading frames, which cover 91% of the 10.1 kb segment. Jia, Y., Slonimski, P.P., Herbert, C.J. Yeast (1991) [Pubmed]
  15. Molecular cloning and biosynthetic regulation of cry1 gene of Saccharomyces cerevisiae. Himmelfarb, H.J., Vassarotti, A., Friesen, J.D. Mol. Gen. Genet. (1984) [Pubmed]
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