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Gene Review

NPL4  -  Npl4p

Saccharomyces cerevisiae S288c

Synonyms: HMG-CoA reductase degradation protein 4, HRD4, Nuclear protein localization protein 4, YBR1231, YBR170C
 
 
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High impact information on NPL4

  • Therefore, Cdc48/p97-Ufd1-Npl4 is an essential chaperone that regulates transformation of the microtubule structure as cells reenter interphase [1].
  • Remarkably, this enzyme binds preferentially ubiquitinated substrates, suggesting that CDC48(UFD1/NPL4) is qualified to selectively remove ubiquitin conjugates from protein complexes [2].
  • Formation of a closed NE requires the p97-Ufd1-Npl4 complex, not previously implicated in membrane fusion [3].
  • Here, we have studied the mechanism by which the p97-Ufd1-Npl4 complex functions in this retrotranslocation pathway [4].
  • We found that CDC48(UFD1/NPL4) plays a second role in the OLE pathway by mediating ERAD of OLE1 [5].
 

Biological context of NPL4

 

Anatomical context of NPL4

  • The only functional data available about mammalian Ufd1p is the ability to form a complex with the rat Npl4 protein, a component of the nuclear pore complex [7].
  • Nuclear transport defects and nuclear envelope alterations are associated with mutation of the Saccharomyces cerevisiae NPL4 gene [9].
  • Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains [4].
 

Other interactions of NPL4

  • We also found that each member of the Cdc48p-Ufd1p-Npl4p complex is individually required for ERAD [6].
  • In addition, we demonstrate that polyubiquitinated Mga2p120 accumulates in cells lacking Npl4p or proteasome function and Rsp5p is required for Mga2p120 polyubiquitination [10].
  • By determining the differential abundance of ubiquitinated proteins in yeast mutated for NPL4 and UBC7, which are major components of ER-associated degradation (ERAD), we furthermore were able to classify 83 of these identified ubiquitinated membrane proteins as potential endogenous substrates of the ERAD pathway [11].
  • In combination, our characterization of GET3 genetic and biochemical interactions with NPL4, GET1, and GET2 implicates Get3 in multiple membrane-dependent pathways [12].
  • Cdc48-Ufd1-Npl4: stuck in the middle with Ub [13].

References

  1. The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis. Cao, K., Nakajima, R., Meyer, H.H., Zheng, Y. Cell (2003) [Pubmed]
  2. Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. Rape, M., Hoppe, T., Gorr, I., Kalocay, M., Richly, H., Jentsch, S. Cell (2001) [Pubmed]
  3. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. Hetzer, M., Meyer, H.H., Walther, T.C., Bilbao-Cortes, D., Warren, G., Mattaj, I.W. Nat. Cell Biol. (2001) [Pubmed]
  4. Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains. Ye, Y., Meyer, H.H., Rapoport, T.A. J. Cell Biol. (2003) [Pubmed]
  5. Role of the ubiquitin-selective CDC48(UFD1/NPL4 )chaperone (segregase) in ERAD of OLE1 and other substrates. Braun, S., Matuschewski, K., Rape, M., Thoms, S., Jentsch, S. EMBO J. (2002) [Pubmed]
  6. HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins. Bays, N.W., Wilhovsky, S.K., Goradia, A., Hodgkiss-Harlow, K., Hampton, R.Y. Mol. Biol. Cell (2001) [Pubmed]
  7. Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L). Botta, A., Tandoi, C., Fini, G., Calabrese, G., Dallapiccola, B., Novelli, G. Gene (2001) [Pubmed]
  8. The Cdc48/p97-Ufd1-Npl4 complex: its potential role in coordinating cellular morphogenesis during the M-G1 transition. Cao, K., Zheng, Y. Cell Cycle (2004) [Pubmed]
  9. Nuclear transport defects and nuclear envelope alterations are associated with mutation of the Saccharomyces cerevisiae NPL4 gene. DeHoratius, C., Silver, P.A. Mol. Biol. Cell (1996) [Pubmed]
  10. Rsp5p is required for ER bound Mga2p120 polyubiquitination and release of the processed/tethered transactivator Mga2p90. Shcherbik, N., Zoladek, T., Nickels, J.T., Haines, D.S. Curr. Biol. (2003) [Pubmed]
  11. A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. Hitchcock, A.L., Auld, K., Gygi, S.P., Silver, P.A. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  12. The Conserved ATPase Get3/Arr4 Modulates the Activity of Membrane-Associated Proteins in Saccharomyces cerevisiae. Auld, K.L., Hitchcock, A.L., Doherty, H.K., Frietze, S., Huang, L.S., Silver, P.A. Genetics (2006) [Pubmed]
  13. Cdc48-Ufd1-Npl4: stuck in the middle with Ub. Bays, N.W., Hampton, R.Y. Curr. Biol. (2002) [Pubmed]
 
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