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Gene Review

UBC7  -  E2 ubiquitin-conjugating protein UBC7

Saccharomyces cerevisiae S288c

Synonyms: DER2, QRI8, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2 7, Ubiquitin-conjugating enzyme E2-18 kDa, ...
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High impact information on QRI8

  • Entry into anaphase and exit from mitosis depend on a ubiquitin-protein ligase complex called the anaphase-promoting complex (APC) or cyclosome [1].
  • Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication [1].
  • Doa10 functions with two E2s, Ubc6 and Ubc7, to ubiquitinate Deg1-bearing substrates, and it is also required for the degradation of at least one ER membrane protein [2].
  • The S. cerevisiae SCFCdc4p ubiquitin-protein ligase complex promotes cell cycle transitions through degradation of cell cycle regulators [3].
  • Ubiquitin conjugation during endoplasmic-reticulum-associated degradation (ERAD) depends on the activity of Ubc7 [4].

Biological context of QRI8

  • The clone (designated UBC7) was confirmed as encoding E2(20 kDa) by comparison of the derived amino acid sequence with peptide sequences of E2(20 kDa) tryptic fragments [5].
  • Cells harboring only the smm1 mutation themselves display temperature-sensitive growth and aberrant mitochondrial inheritance and morphology at the nonpermissive temperature. smm1 maps to RSP5, a gene encoding an essential ubiquitin-protein ligase [6].
  • Rsp5p is an ubiquitin-protein ligase of Saccharomyces cerevisiae that has been implicated in numerous processes including transcription, mitochondrial inheritance, and endocytosis [7].
  • In addition, we have mapped UBC7 to human chromosome 21q22.3 close to 21qtel [8].

Anatomical context of QRI8

  • Yeast strains lacking UBC7, CUE1, or DOA10 also assembled aberrant karmellae (ordered arrays of membranes surrounding the nucleus that assemble when HMGR is expressed at high levels) [9].
  • Membrane topology and function of Der3/Hrd1p as a ubiquitin-protein ligase (E3) involved in endoplasmic reticulum degradation [10].
  • Ubc7Deltaloop had higher affinity for the E1-Ub thiol ester than native UBC7 and permitted conjugation of Ub to selected proteins in the testis but did not permit the broad spectrum E3-dependent conjugation to endogenous reticulocyte proteins [11].

Associations of QRI8 with chemical compounds

  • Cells lacking UBC7, CUE1, or DOA10 were cold sensitive, suggesting that these ERAD proteins have a role in cold adaptation, perhaps through effects on sterol biosynthesis [9].
  • Overexpression of UBC4 and of UBC7, two other genes for ubiquitin-conjugating enzymes, also conferred resistance to methylmercury [12].
  • Selective solubilization of the protein using 5 M urea followed by dialysis, MonoQ FPLC, and Superdex-75 FPLC yielded electrophoretically pure Ubc7 protein [13].

Other interactions of QRI8

  • QRI8, a novel ubiquitin-conjugating enzyme in Saccharomyces cerevisiae [14].
  • The C-terminal extensions of these clones, which vary in length from 16 to 50 amino acid residues, are presumed to contain degradation signals channeling proteins for degradation via the UBC6 and/or UBC7 subpathways of the ubiquitin system [15].
  • By determining the differential abundance of ubiquitinated proteins in yeast mutated for NPL4 and UBC7, which are major components of ER-associated degradation (ERAD), we furthermore were able to classify 83 of these identified ubiquitinated membrane proteins as potential endogenous substrates of the ERAD pathway [16].
  • Hmg2p degradation required the UBC7 gene, and Hmg2p protein was directly ubiquitinated [17].
  • Only two genes, UBC7 and YAL011W, were identified in all three experiments [18].

Analytical, diagnostic and therapeutic context of QRI8


  1. Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Zachariae, W., Shevchenko, A., Andrews, P.D., Ciosk, R., Galova, M., Stark, M.J., Mann, M., Nasmyth, K. Science (1998) [Pubmed]
  2. A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matalpha2 repressor degradation. Swanson, R., Locher, M., Hochstrasser, M. Genes Dev. (2001) [Pubmed]
  3. Ubiquitination and degradation of the substrate recognition subunits of SCF ubiquitin-protein ligases. Zhou, P., Howley, P.M. Mol. Cell (1998) [Pubmed]
  4. A regulatory link between ER-associated protein degradation and the unfolded-protein response. Friedlander, R., Jarosch, E., Urban, J., Volkwein, C., Sommer, T. Nat. Cell Biol. (2000) [Pubmed]
  5. Cloning and characterization of a 20-kDa ubiquitin carrier protein from wheat that catalyzes multiubiquitin chain formation in vitro. Van Nocker, S., Vierstra, R.D. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  6. A role for ubiquitination in mitochondrial inheritance in Saccharomyces cerevisiae. Fisk, H.A., Yaffe, M.P. J. Cell Biol. (1999) [Pubmed]
  7. Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae. Kamińska, J., Gajewska, B., Hopper, A.K., Zoładek, T. Mol. Cell. Biol. (2002) [Pubmed]
  8. Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae ubc7 gene. Katsanis, N., Fisher, E.M. Genomics (1998) [Pubmed]
  9. Endoplasmic reticulum-associated degradation is required for cold adaptation and regulation of sterol biosynthesis in the yeast Saccharomyces cerevisiae. Loertscher, J., Larson, L.L., Matson, C.K., Parrish, M.L., Felthauser, A., Sturm, A., Tachibana, C., Bard, M., Wright, R. Eukaryotic Cell (2006) [Pubmed]
  10. Membrane topology and function of Der3/Hrd1p as a ubiquitin-protein ligase (E3) involved in endoplasmic reticulum degradation. Deak, P.M., Wolf, D.H. J. Biol. Chem. (2001) [Pubmed]
  11. Identification of rabbit reticulocyte E217K as a UBC7 homologue and functional characterization of its core domain loop. Lin, H., Wing, S.S. J. Biol. Chem. (1999) [Pubmed]
  12. Overexpression of the ubiquitin-conjugating enzyme Cdc34 confers resistance to methylmercury in Saccharomyces cerevisiae. Furuchi, T., Hwang, G.W., Naganuma, A. Mol. Pharmacol. (2002) [Pubmed]
  13. Bacterial expression of the Saccharomyces cerevisiae ubiquitin-conjugating enzyme Ubc7. Yamazaki, R.K., Chau, V. Protein Expr. Purif. (1996) [Pubmed]
  14. QRI8, a novel ubiquitin-conjugating enzyme in Saccharomyces cerevisiae. Vassal, A., Boulet, A., Decoster, E., Faye, G. Biochim. Biophys. Acta (1992) [Pubmed]
  15. Degradation signals for ubiquitin system proteolysis in Saccharomyces cerevisiae. Gilon, T., Chomsky, O., Kulka, R.G. EMBO J. (1998) [Pubmed]
  16. A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. Hitchcock, A.L., Auld, K., Gygi, S.P., Silver, P.A. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  17. Ubiquitin-mediated regulation of 3-hydroxy-3-methylglutaryl-CoA reductase. Hampton, R.Y., Bhakta, H. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  18. Parallel analysis of tagged deletion mutants efficiently identifies genes involved in endoplasmic reticulum biogenesis. Wright, R., Parrish, M.L., Cadera, E., Larson, L., Matson, C.K., Garrett-Engele, P., Armour, C., Lum, P.Y., Shoemaker, D.D. Yeast (2003) [Pubmed]
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