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Gene Review

RTF1  -  Rtf1p

Saccharomyces cerevisiae S288c

Synonyms: CSL3, HRA458, RNA polymerase-associated protein RTF1, YGL244W
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High impact information on RTF1

  • We identified NAB3 as a dosage suppressor of rtf1 [1].
  • To elucidate the functions of this complex, we isolated missense mutations in the gene encoding the Rtf1 subunit and used them to identify functionally interacting proteins [1].
  • Finally, we demonstrate that Chd1, Rtf1 and Spt5 associate with actively transcribed regions of chromatin [2].
  • Using RNAi, we examined the role of Rtf1 in histone methylation and gene expression in Drosophila melanogaster [3].
  • Furthermore, we show that RNAi-mediated reduction of dRtf1 results in a reduction in histone H3K4 trimethylation levels on bulk histones and chromosomes in vivo, indicating that the histone modification pathway via Rtf1 is conserved among yeast, Drosophila, and human [3].

Biological context of RTF1


Associations of RTF1 with chemical compounds


Physical interactions of RTF1

  • To uncover additional factors that regulate chromatin or transcription, we performed a genetic screen for mutations that cause lethality in the absence of the Paf1 complex component Rtf1 [7].
  • Taken together, our findings suggest that Rtf1 either directly or indirectly regulates the DNA binding properties of TBP and, consequently, the relative activities of different TATA elements in vivo [8].

Regulatory relationships of RTF1

  • Together, our results indicate that Rtf1 influences transcription and chromatin structure through several independent functional domains and that Rtf1 may function independently of its association with other members of the Paf1 complex [9].

Other interactions of RTF1


Analytical, diagnostic and therapeutic context of RTF1


  1. A Requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation. Sheldon, K.E., Mauger, D.M., Arndt, K.M. Mol. Cell (2005) [Pubmed]
  2. Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes. Simic, R., Lindstrom, D.L., Tran, H.G., Roinick, K.L., Costa, P.J., Johnson, A.D., Hartzog, G.A., Arndt, K.M. EMBO J. (2003) [Pubmed]
  3. Drosophila Rtf1 functions in histone methylation, gene expression, and Notch signaling. Tenney, K., Gerber, M., Ilvarsonn, A., Schneider, J., Gause, M., Dorsett, D., Eissenberg, J.C., Shilatifard, A. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  4. Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mueller, C.L., Jaehning, J.A. Mol. Cell. Biol. (2002) [Pubmed]
  5. The Rtf1 component of the Paf1 transcriptional elongation complex is required for ubiquitination of histone H2B. Ng, H.H., Dole, S., Struhl, K. J. Biol. Chem. (2003) [Pubmed]
  6. Synthetic lethal interactions suggest a role for the Saccharomyces cerevisiae Rtf1 protein in transcription elongation. Costa, P.J., Arndt, K.M. Genetics (2000) [Pubmed]
  7. Identification of Rkr1, a Nuclear RING Domain Protein with Functional Connections to Chromatin Modification in Saccharomyces cerevisiae. Braun, M.A., Costa, P.J., Crisucci, E.M., Arndt, K.M. Mol. Cell. Biol. (2007) [Pubmed]
  8. Identification of RTF1, a novel gene important for TATA site selection by TATA box-binding protein in Saccharomyces cerevisiae. Stolinski, L.A., Eisenmann, D.M., Arndt, K.M. Mol. Cell. Biol. (1997) [Pubmed]
  9. Rtf1 is a multifunctional component of the Paf1 complex that regulates gene expression by directing cotranscriptional histone modification. Warner, M.H., Roinick, K.L., Arndt, K.M. Mol. Cell. Biol. (2007) [Pubmed]
  10. Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization. Porter, S.E., Penheiter, K.L., Jaehning, J.A. Eukaryotic Cell (2005) [Pubmed]
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