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Gene Review

VPS35  -  Vps35p

Saccharomyces cerevisiae S288c

Synonyms: GRD9, J0580, VPT7, Vacuolar protein sorting-associated protein 35, Vacuolar protein-targeting protein 7, ...
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High impact information on VPS35

  • Furthermore, mutations in the cytosolic domains of A-ALP and another cargo protein, Vps10p, were identified that suppressed cargo-specific mutations in Vps35p but did not suppress the retrieval defects of a vps35 null mutation [1].
  • Furthermore, gel filtration analysis indicates that Vps35p and Vps5p are present on a population of vesicles and tubules slightly larger than COPI/coatomer-coated vesicles [2].
  • Density gradient fractionation of Golgi/endosomal/vesicular membranes reveals that Vps35p cofractionates with Vps5p/Vps17p in a vesicle-enriched dense membrane fraction [2].
  • The route that Vps10p takes to reach the vacuole in a vps35 mutant does not depend upon Sec1p mediated arrival at the plasma membrane but does require the activity of the pre-vacuolar endosomal t-SNARE, Pep12p [3].
  • We cloned the Arabidopsis thaliana homologs of the three yeast proteins (Vps35, Vps29, and Vps26) constituting the larger subunit of retromer and prepared antisera against them [4].

Biological context of VPS35

  • This phenotype is similar to that seen in cells with mutations in the previously characterized VPS10 and VPS35 genes [3].
  • We have cloned the wild-type VPS35 gene by complementation of the vacuolar protein sorting defect exhibited by the vps35-17 mutant [5].
  • Disruption of the VPS35 gene demonstrated that it is not essential for yeast cell growth [5].
  • Sequencing of an open reading frame 450 bp downstream from the yeast VPS35 gene revealed a putative peptide of 452 amino acids and 52.7 kDa [6].

Physical interactions of VPS35

  • Vps35p is required for the prevacuolar compartment-to-TGN transport of both A-ALP and Vps10p [7].

Other interactions of VPS35

  • A temperature conditional allele of the VPS35 gene was generated and has been found to cause missorting/secretion of CPY and also Vps10p to mislocalize to a vacuolar membrane fraction at the nonpermissive temperature [3].
  • These results indicate that the vps26(+), vps29(+) and vps35(+) gene products are required for retrograde transport of SpVps10p from the prevacuolar compartment back to the Golgi in Sch. pombe cells [8].


  1. Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p. Nothwehr, S.F., Ha, S.A., Bruinsma, P. J. Cell Biol. (2000) [Pubmed]
  2. A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. Seaman, M.N., McCaffery, J.M., Emr, S.D. J. Cell Biol. (1998) [Pubmed]
  3. Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products. Seaman, M.N., Marcusson, E.G., Cereghino, J.L., Emr, S.D. J. Cell Biol. (1997) [Pubmed]
  4. Plant retromer, localized to the prevacuolar compartment and microvesicles in Arabidopsis, may interact with vacuolar sorting receptors. Oliviusson, P., Heinzerling, O., Hillmer, S., Hinz, G., Tse, Y.C., Jiang, L., Robinson, D.G. Plant Cell (2006) [Pubmed]
  5. Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases. Paravicini, G., Horazdovsky, B.F., Emr, S.D. Mol. Biol. Cell (1992) [Pubmed]
  6. The yeast FBP26 gene codes for a fructose-2,6-bisphosphatase. Paravicini, G., Kretschmer, M. Biochemistry (1992) [Pubmed]
  7. Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment. Nothwehr, S.F., Bruinsma, P., Strawn, L.A. Mol. Biol. Cell (1999) [Pubmed]
  8. Vacuolar protein sorting receptor in Schizosaccharomyces pombe. Iwaki, T., Hosomi, A., Tokudomi, S., Kusunoki, Y., Fujita, Y., Giga-Hama, Y., Tanaka, N., Takegawa, K. Microbiology (Reading, Engl.) (2006) [Pubmed]
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