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MCD4  -  Mcd4p

Saccharomyces cerevisiae S288c

Synonyms: FSR2, GPI ethanolamine phosphate transferase 1, Morphogenesis checkpoint-dependent protein 4, SSU21, Supersecretion of u-PA protein 21, ...
 
 
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High impact information on MCD4

  • MCD4 is essential because Gpi10p, the mannosyltransferase adding the subsequent alpha1-2-linked mannose, requires substrates with an ethanolaminephosphate on the alpha1-4-linked mannose [1].
  • In yeast, the ethanolaminephosphate on the alpha1-4-linked mannose is added during the biosynthesis of the GPI lipid by Mcd4p [1].
  • Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi [1].
  • The three putative ethanolamine-phosphate transferases Gpi13p, Gpi7p, and Mcd4p cannot replace each other even when overexpressed [2].
  • We developed a functional screen for ATP release to the extracellular space and identified Mcd4p, a 919-amino acid membrane protein with 14 putative transmembrane domains, as a participant in glucose-dependent ATP release from Saccharomyces cerevisiae [3].
 

Biological context of MCD4

  • Here we investigate whether YLL031c, an open reading frame predicting a further homologue of GPI7 and MCD4, plays any role in GPI anchoring [4].
  • The other group of fluphenazine-resistant mutants, carrying fsr2 mutations, showed Ca2+-dependent growth at 35 degrees C. Growth of the fsr2 mutants at 35 degrees C was arrested at the G2 stage of the cell cycle in Ca2+-poor medium [5].
 

Anatomical context of MCD4

  • These observations suggest that Mpc1 preferentially utilizes phosphatidylethanolamine produced by Psd2 that is localized in Golgi/vacuole. fsr2-1 dpl1 Delta psd1delta strains showed slower growth than fsr2-1 dpl1delta psd2 delta, suggesting that Fsr2 enzyme depends more on Dpl1 and Psd1 for production of phosphatidylethanolamine [6].
  • The deletion of MCD4 leads to an increase in beta-1,6-glucan level and a decrease in glycosylphosphatidylinositol-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae, suggesting that mcd4 deletion mutant (mcd4Delta) displays beta-glucans on the cell surface without a mannan cover [7].
  • It is likely that the Mcd4p pathway is generally involved in non-mitochondrial ATP movement across membranes, it is essential for Golgi and endoplasmic reticulum function, and its occurrence led to the appearance of P2 purinergic receptors [3].
 

Associations of MCD4 with chemical compounds

  • A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis [8].
 

Other interactions of MCD4

References

  1. Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi. Zhu, Y., Vionnet, C., Conzelmann, A. J. Biol. Chem. (2006) [Pubmed]
  2. Glycosylphosphatidylinositol (GPI) proteins of Saccharomyces cerevisiae contain ethanolamine phosphate groups on the alpha1,4-linked mannose of the GPI anchor. Imhof, I., Flury, I., Vionnet, C., Roubaty, C., Egger, D., Conzelmann, A. J. Biol. Chem. (2004) [Pubmed]
  3. ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase. Zhong, X., Malhotra, R., Guidotti, G. J. Biol. Chem. (2003) [Pubmed]
  4. YLL031c belongs to a novel family of membrane proteins involved in the transfer of ethanolaminephosphate onto the core structure of glycosylphosphatidylinositol anchors in yeast. Flury, I., Benachour, A., Conzelmann, A. J. Biol. Chem. (2000) [Pubmed]
  5. Fluphenazine-resistant Saccharomyces cerevisiae mutants defective in the cell division cycle. Matsumoto, K., Uno, I., Ishikawa, T. J. Bacteriol. (1986) [Pubmed]
  6. Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae. Toh-e, A., Oguchi, T. Genes Genet. Syst. (2002) [Pubmed]
  7. Saccharomyces cerevisiae mutant displaying beta-glucans on cell surface. Sakai, Y., Azuma, M., Takada, Y., Umeyama, T., Kaneko, A., Fujita, T., Igarashi, K., Ooshima, H. J. Biosci. Bioeng. (2007) [Pubmed]
  8. A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis. Storey, M.K., Wu, W.I., Voelker, D.R. Biochim. Biophys. Acta (2001) [Pubmed]
  9. Its8, a fission yeast homolog of Mcd4 and Pig-n, is involved in GPI anchor synthesis and shares an essential function with calcineurin in cytokinesis. Yada, T., Sugiura, R., Kita, A., Itoh, Y., Lu, Y., Hong, Y., Kinoshita, T., Shuntoh, H., Kuno, T. J. Biol. Chem. (2001) [Pubmed]
 
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