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Gene Review:

THI80 - thiamine diphosphokinase

Saccharomyces cerevisiae S288c

Synonyms: TPK, Thiamine kinase, Thiamine pyrophosphokinase, YOR143C, YOR3373C

Nosaka, K. et al., Iwashima, A. et al., Nishimura, H. et al., Fankhauser, H. et al., Baker, L.J. et al.

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High impact information on THI80

Schizosaccharomyces pombe thiamine pyrophosphokinase is encoded by gene tnr3 and is a regulator of thiamine metabolism, phosphate metabolism, mating, and growth [1].
Studies of Northern blot analysis and the enzyme assay demonstrated that the THI80 gene expression is regulated mainly at the mRNA level by the intracellular thiamin pyrophosphate and requires the positive regulatory factors encoded by THI2 and THI3 genes [2].
The mutant shows markedly reduced activity of thiamine pyrophosphokinase (EC 2.7.6.2) and high resistance to oxythiamine, a thiamine antagonist whose potency depends on thiamine pyrophosphokinase activity [3].
TPK has no sequence homologs in the PDB and functions by an unknown mechanism [4].
The TPK dimer contains two compound active sites at the subunit interface [4].

Biological context of THI80

The results strongly support the supposition that thiamine as well as dimethialium can be transported and accumulated without obligatory phosphorylation in yeast cells, since dimethialium is not phosphorylated by yeast thiamine pyrophosphokinase [5].

Associations of THI80 with chemical compounds

Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae [2].

References

Schizosaccharomyces pombe thiamine pyrophosphokinase is encoded by gene tnr3 and is a regulator of thiamine metabolism, phosphate metabolism, mating, and growth. Fankhauser, H., Zurlinden, A., Schweingruber, A.M., Edenharter, E., Schweingruber, M.E. J. Biol. Chem. (1995) [Pubmed]
Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae. Nosaka, K., Kaneko, Y., Nishimura, H., Iwashima, A. J. Biol. Chem. (1993) [Pubmed]
A constitutive thiamine metabolism mutation, thi80, causing reduced thiamine pyrophosphokinase activity in Saccharomyces cerevisiae. Nishimura, H., Kawasaki, Y., Nosaka, K., Kaneko, Y., Iwashima, A. J. Bacteriol. (1991) [Pubmed]
The crystal structure of yeast thiamin pyrophosphokinase. Baker, L.J., Dorocke, J.A., Harris, R.A., Timm, D.E. Structure (Camb.) (2001) [Pubmed]
Active transport of dimethialium in Saccharomyces cerevisiae. Iwashima, A., Nishimura, H., Sempuku, K. Experientia (1980) [Pubmed]

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AGOS_AFR387C	Ashbya gossypii ATCC 10895
KLLA0E03257g	Kluyveromyces lactis NRRL Y-1140

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