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RPC34  -  Rpc34p

Saccharomyces cerevisiae S288c

Synonyms: C34, DNA-directed RNA polymerase III 36 kDa polypeptide, DNA-directed RNA polymerase III subunit RPC6, N2031, RNA polymerase III subunit C6, ...
 
 
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High impact information on RPC34

  • Finally, we demonstrate that BRF but not TFIIB binds the Pol III subunit C34 and we define a region of C34 necessary for this interaction [1].
  • Antibody labeling experiments localize the C82/C34/C31 subcomplex to the clamp side of the DNA binding cleft, consistent with its known role in transcription initiation [2].
  • TFIIIB70 interacts with TBP, a unique pol III subunit, C34, and the 131-kDa subunit of the pol III-specific complex, TFIIIC [3].
  • Transcription of the tRNAGlu3 gene in vitro was inhibited when RNA polymerase C was preincubated with antibodies directed to C128, C82, C53, C34, C23, or C19 [4].
  • In contrast, C34 and A34 were not transformed at all neither in the cytoplasm nor in the nucleus of the frog oocyte [5].
 

Biological context of RPC34

  • Mutagenesis of yeast TFIIIB70 reveals C-terminal residues critical for interaction with TBP and C34 [6].
  • Aminoacylation analyses of these tRNA transcripts with yeast tyrosyl-tRNA synthetase revealed that the replacement of G34 by C34 (mutation to amber suppressor) severely impaired the aminoacylation, whereas the replacement of the U4:G69 wobble base-pair in the acceptor stem region by C4:G69 normal Watson-Crick type base-pair improved it [7].
 

Associations of RPC34 with chemical compounds

  • Nevertheless, in the anticodon loop, only two interesting differences are present: A37 is not modified while C34 at the first anticodon position is modified into a ribose 2'-O methyl derivative (Cm) [8].
 

Other interactions of RPC34

  • Our results underscore the important role of region II of TFIIIB70 in pre-initiation as well as transcription complex assembly via C34 and TBP binding [6].
  • The C31 subunit belongs to a complex of three subunits (C31, C34 and C82) specific to RNA polymerase (pol) III that have no counterparts in other RNA polymerases [9].
  • This complex is thought to play a role in transcription initiation since it interacts with the general initiation factor TFIIIB via subunit C34 [9].
  • Antibodies to C82, C53, and C34 were much less inhibitory in the nonspecific assay [4].

References

  1. Conserved functional domains of the RNA polymerase III general transcription factor BRF. Khoo, B., Brophy, B., Jackson, S.P. Genes Dev. (1994) [Pubmed]
  2. Insights into Transcription Initiation and Termination from the Electron Microscopy Structure of Yeast RNA Polymerase III. Fernández-Tornero, C., Böttcher, B., Riva, M., Carles, C., Steuerwald, U., Ruigrok, R.W., Sentenac, A., Müller, C.W., Schoehn, G. Mol. Cell (2007) [Pubmed]
  3. Expression and purification of the RNA polymerase III transcription specificity factor IIIB70 from Saccharomyces cerevisiae and its cooperative binding with TATA-binding protein. Librizzi, M.D., Moir, R.D., Brenowitz, M., Willis, I.M. J. Biol. Chem. (1996) [Pubmed]
  4. Yeast RNA polymerase C and its subunits. Specific antibodies as structural and functional probes. Huet, J., Riva, M., Sentenac, A., Fromageot, P. J. Biol. Chem. (1985) [Pubmed]
  5. Enzymatic replacement in vitro of the first anticodon base of yeast tRNAAsp: application to the study of tRNA maturation in vivo, after microinjection into frog oocytes. Carbon, P., Haumont, E., De Henau, S., Keith, G., Grosjean, H. Nucleic Acids Res. (1982) [Pubmed]
  6. Mutagenesis of yeast TFIIIB70 reveals C-terminal residues critical for interaction with TBP and C34. Andrau, J.C., Sentenac, A., Werner, M. J. Mol. Biol. (1999) [Pubmed]
  7. Use of RNase P for Efficient Preparation of Yeast tRNATyr Transcript and Its Mutants. Fukunaga, J., Gouda, M., Umeda, K., Ohno, S., Yokogawa, T., Nishikawa, K. J. Biochem. (2006) [Pubmed]
  8. Temperature jump relaxation studies on the interactions between transfer RNAs with complementary anticodons. The effect of modified bases adjacent to the anticodon triplet. Houssier, C., Grosjean, H. J. Biomol. Struct. Dyn. (1985) [Pubmed]
  9. A mutation in the C31 subunit of Saccharomyces cerevisiae RNA polymerase III affects transcription initiation. Thuillier, V., Stettler, S., Sentenac, A., Thuriaux, P., Werner, M. EMBO J. (1995) [Pubmed]
 
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