The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

PUS1  -  pseudouridine synthase PUS1

Saccharomyces cerevisiae S288c

Synonyms: YPL212C, tRNA pseudouridine synthase 1, tRNA pseudouridylate synthase 1, tRNA-uridine isomerase 1
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of PUS1

 

High impact information on PUS1

 

Biological context of PUS1

  • Furthermore, nuclear export of at least one wild-type tRNA species becomes defective in the absence of Pus1p [5].
  • Yeast Pus1p catalyzes the formation of pseudouridine (psi) at specific sites of several tRNAs, but its function is not essential for cell viability [5].
  • Our data, thus, show that the modifications formed by Pus1p are essential when other aspects of tRNA biogenesis or function are compromised and suggest that impairment of nuclear tRNA export in the absence of Pus1p might contribute to this phenotype [5].
  • The presence of a G26.A44 base-pair in tRNA increases its association constant rate with Pus1 (ka) by a factor of approximately 100, resulting in a decrease of the overall equilibrium dissociation constant (Kd) [1].
  • Comparison of the deduced amino acid sequence of DEG1 with a protein sequence databank revealed homology with the enzyme tRNA pseudouridine synthase I of E. coli [6].
 

Associations of PUS1 with chemical compounds

  • Mapping of pseudouridine residues present in various tRNAs extracted from the PUS1-disrupted strain confirms the in vitro data obtained with the recombinant Pus1p [7].
  • We show here that Pus1p becomes essential when another tRNA:pseudouridine synthase, Pus4p, or the essential minor tRNA for glutamine are mutated [5].
  • Although dispensable for activity, both the presence of a D-stem-loop and the presence of a G26.A44 base-pair, near the target uridine U27, are important elements for Pus1 tRNA high affinity recognition [1].

References

  1. Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA. Arluison, V., Buckle, M., Grosjean, H. J. Mol. Biol. (1999) [Pubmed]
  2. Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism. Ma, X., Yang, C., Alexandrov, A., Grayhack, E.J., Behm-Ansmant, I., Yu, Y.T. EMBO J. (2005) [Pubmed]
  3. Nuclear pore proteins are involved in the biogenesis of functional tRNA. Simos, G., Tekotte, H., Grosjean, H., Segref, A., Sharma, K., Tollervey, D., Hurt, E.C. EMBO J. (1996) [Pubmed]
  4. Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA. Massenet, S., Motorin, Y., Lafontaine, D.L., Hurt, E.C., Grosjean, H., Branlant, C. Mol. Cell. Biol. (1999) [Pubmed]
  5. Pus1p-dependent tRNA pseudouridinylation becomes essential when tRNA biogenesis is compromised in yeast. Grosshans, H., Lecointe, F., Grosjean, H., Hurt, E., Simos, G. J. Biol. Chem. (2001) [Pubmed]
  6. A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae. Carbone, M.L., Solinas, M., Sora, S., Panzeri, L. Curr. Genet. (1991) [Pubmed]
  7. The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity. Motorin, Y., Keith, G., Simon, C., Foiret, D., Simos, G., Hurt, E., Grosjean, H. RNA (1998) [Pubmed]
 
WikiGenes - Universities