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Gene Review

NSP1  -  Nsp1p

Saccharomyces cerevisiae S288c

Synonyms: J1207, Nuclear pore protein NSP1, Nucleoporin NSP1, Nucleoskeletal-like protein, YJL041W, ...
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Disease relevance of NSP1

  • To investigate the molecular basis underlying complex formation, recombinant full-length Nup49p and Nup57p and the carboxyl-terminal domain of Nsp1p, which lacks the FXFG repeat domain, were expressed in Escherichia coli [1].

High impact information on NSP1

  • An essential yeast protein, Nup145p, is identified via its genetic interaction with the nucleoporin Nsp1p [2].
  • The 85 kd subunit (p85 alpha) lacks Pl3-kinase activity and acts as an adaptor, coupling the 110 kd subunit (p110) to activated protein tyrosine kinases [3].
  • The central domain of NUP1 consists of a series of degenerate repeats similar to those found in the nucleoskeletal protein NSP1, a protein that cross-reacts with monoclonal antibodies against NUP1 [4].
  • Employing immunoelectron microscopy on yeast cells, we show that NSP1 is located at the nuclear pores [5].
  • NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain [5].

Biological context of NSP1

  • The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport [6].
  • NSP1 is located at the nuclear periphery in yeast and is essential for cell growth [5].
  • The molecular analysis of the NSP1 protein points to a two domain model: a nonessential domain (the first 603 amino acids) composed of repetitive sequences common to other nuclear proteins and an essential, carboxy-terminal domain (residues 604-823) mediating the vital function of NSP1 [5].
  • To further analyze the structural and functional similarity between NSP1 and mammalian nucleoporins, we cloned and sequenced nucleoporin p62 from a HeLa cDNA library [7].
  • This result suggests that NSP1 is required for the coordinated cell cycle in S. cerevisiae [8].

Anatomical context of NSP1


Associations of NSP1 with chemical compounds


Physical interactions of NSP1


Regulatory relationships of NSP1


Other interactions of NSP1

  • Conversely, affinity purification of tagged NSP49 enriches for NSP1, the p54 and the NIC96 component [9].
  • Accordingly, interaction of Nup82p with Nsp1p coil 2 is competed by excess Nup57p [6].
  • Strains mutant for Nup133p or Nsp1p accumulated lower levels of several unspliced pre-tRNAs [24].
  • The Nup82 protein can be divided into at least two different domains both required for the essential function, but it is only the carboxy-terminal domain, exhibiting heptad repeats, which binds to Nsp1p [20].
  • Decreased levels of NPC-associated Nsp1p and Nup116p were observed [25].

Analytical, diagnostic and therapeutic context of NSP1


  1. In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p. Schlaich, N.L., Häner, M., Lustig, A., Aebi, U., Hurt, E.C. Mol. Biol. Cell (1997) [Pubmed]
  2. Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif. Fabre, E., Boelens, W.C., Wimmer, C., Mattaj, I.W., Hurt, E.C. Cell (1994) [Pubmed]
  3. Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit. Hiles, I.D., Otsu, M., Volinia, S., Fry, M.J., Gout, I., Dhand, R., Panayotou, G., Ruiz-Larrea, F., Thompson, A., Totty, N.F. Cell (1992) [Pubmed]
  4. The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Davis, L.I., Fink, G.R. Cell (1990) [Pubmed]
  5. NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Nehrbass, U., Kern, H., Mutvei, A., Horstmann, H., Marshallsay, B., Hurt, E.C. Cell (1990) [Pubmed]
  6. The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport. Bailer, S.M., Balduf, C., Hurt, E. Mol. Cell. Biol. (2001) [Pubmed]
  7. Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Carmo-Fonseca, M., Kern, H., Hurt, E.C. Eur. J. Cell Biol. (1991) [Pubmed]
  8. NSP1, a yeast protein located at the nuclear periphery, is required for the cell cycle of Saccharomyces cerevisiae. Hurt, E.C. J. Cell Sci. Suppl. (1989) [Pubmed]
  9. Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. Grandi, P., Doye, V., Hurt, E.C. EMBO J. (1993) [Pubmed]
  10. NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex. Loeb, J.D., Davis, L.I., Fink, G.R. Mol. Biol. Cell (1993) [Pubmed]
  11. Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins. Aris, J.P., Blobel, G. J. Cell Biol. (1989) [Pubmed]
  12. Reconstitution of nuclear protein transport with semi-intact yeast cells. Schlenstedt, G., Hurt, E., Doye, V., Silver, P.A. J. Cell Biol. (1993) [Pubmed]
  13. Wortmannin-sensitive trafficking pathways in Chinese hamster ovary cells. Differential effects on endocytosis and lysosomal sorting. Martys, J.L., Wjasow, C., Gangi, D.M., Kielian, M.C., McGraw, T.E., Backer, J.M. J. Biol. Chem. (1996) [Pubmed]
  14. Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p. Clarkson, W.D., Kent, H.M., Stewart, M. J. Mol. Biol. (1996) [Pubmed]
  15. Phosphatidylinositol 3-kinase activation is mediated by high-affinity interactions between distinct domains within the p110 and p85 subunits. Holt, K.H., Olson, L., Moye-Rowley, W.S., Pessin, J.E. Mol. Cell. Biol. (1994) [Pubmed]
  16. Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. Stack, J.H., Emr, S.D. J. Biol. Chem. (1994) [Pubmed]
  17. Characterization of the p33 subunit of eukaryotic translation initiation factor-3 from Saccharomyces cerevisiae. Hanachi, P., Hershey, J.W., Vornlocher, H.P. J. Biol. Chem. (1999) [Pubmed]
  18. Modulation of the substrate specificity of the mammalian phosphatidylinositol 3-kinase by cholesterol sulfate and sulfatide. Woscholski, R., Kodaki, T., Palmer, R.H., Waterfield, M.D., Parker, P.J. Biochemistry (1995) [Pubmed]
  19. Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. Grandi, P., Schlaich, N., Tekotte, H., Hurt, E.C. EMBO J. (1995) [Pubmed]
  20. A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. Grandi, P., Emig, S., Weise, C., Hucho, F., Pohl, T., Hurt, E.C. J. Cell Biol. (1995) [Pubmed]
  21. Functional characterization of a Nup159p-containing nuclear pore subcomplex. Belgareh, N., Snay-Hodge, C., Pasteau, F., Dagher, S., Cole, C.N., Doye, V. Mol. Biol. Cell (1998) [Pubmed]
  22. The small GTPase Gsp1p binds to the repeat domain of the nucleoporin Nsp1p. Stochaj, U., Héjazi, M., Belhumeur, P. Biochem. J. (1998) [Pubmed]
  23. NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation. Mutvei, A., Dihlmann, S., Herth, W., Hurt, E.C. Eur. J. Cell Biol. (1992) [Pubmed]
  24. Yeast nucleoporin mutants are defective in pre-tRNA splicing. Sharma, K., Fabre, E., Tekotte, H., Hurt, E.C., Tollervey, D. Mol. Cell. Biol. (1996) [Pubmed]
  25. A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly. Bucci, M., Wente, S.R. Mol. Biol. Cell (1998) [Pubmed]
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