Disease relevance of CSR2

• Molecular analysis revealed that the region responsible for the suppression of galactose toxicity corresponds to the ORF YPR030w, which was named MRG19 [1].

High impact information on CSR2

• Using this screening method, we successfully identified two novel localized mRNAs, CSR2 and DAL81, which showed bud-tip localization [2].
• It was previously shown that MRG19 downregulates carbon metabolism in Saccharomyces cerevisiae upon glucose exhaustion, and that the gene is glucose repressed [3].
• As compared to the wild-type strain, the MRG19 disruptant showed a decrease in the ratio of 2-oxoglutarate to glutamate under nitrogen-limited conditions [3].
• Unlike the effect on GAL gene expression, disruption of MRG19 leads to de-repression of CYC1-driven beta-galactosidase activity [4].
• We have reported previously that multiple copies of MRG19 suppress GAL genes in a wild-type but not in a gal80 strain of Saccharomyces cerevisiae [4].

Biological context of CSR2

• Disruption of MRG19 leads to an increase in the cell density at stationary phase in synthetic complete medium [1].
• MRG19 encodes a previously uncharacterised 124-kDa protein that shows no sequence homology to any known proteins [1].
• Disruption of MRG19 in a gal3 background (this strain shows long-term adaptation phenotype) further delays the GAL induction, supporting the notion that its function is important only under low inducing signals [4].

Associations of CSR2 with chemical compounds

• Based on the above results, we propose that Mrg19p is a regulator of galactose and nonfermentable carbon utilization [4].
• Western blot analysis indicated that MRG19 is a glucose repressible gene and is expressed in galactose and glycerol plus lactate [4].

Regulatory relationships of CSR2

• These results imply that multiple copies of MRG19 suppress galactokinase expression probably at the level of transcription [1].

Other interactions of CSR2

• Galactokinase activity was substantially reduced in cell extracts obtained from transformants bearing multiple copies of MRG19 [1].
• Multiple copies of MRG19 suppress transcription of the GAL1 promoter in a GAL80-dependent manner in Saccharomyces cerevisiae [1].

References