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Gene Review

nusA  -  transcription termination/antitermination...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3158, JW3138
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Disease relevance of nusA


High impact information on nusA

  • The DNA sequence at tL shows striking homologies with trp t', a terminator also strongly affected in vitro by the nusA protein [6].
  • We have determined the effects of the nusA gene protein and the regulatory nucleotide guanosine tetraphosphate (ppGpp) on pausing and termination of transcription in the leader region of the rrnB operon in vitro [6].
  • A completed termination event at tR2 requires both the nusA gene protein and the previously described E. coli termination factor rho [2].
  • In vitro the nusA gene protein causes RNA polymerase to pause in the tR2 terminator region of lambda DNA [2].
  • In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins [7].

Chemical compound and disease context of nusA


Biological context of nusA

  • The nusA gene is immediately followed by another open reading frame encoding a polypeptide of at least 22 amino acids, which was identified as the initial portion of the infB structural gene [1].
  • The genes infB, for translational initiation factor IF2, and nusA for a protein involved in transcription termination are carried on a 4.8 X 10(3) base-pair DNA fragment [11].
  • Transcriptional polarity in rRNA operons of Escherichia coli nusA and nusB mutant strains [12].
  • We compared the promoter region of the metZ gene with that of the metY gene, which encodes the tRNA(f2Met) and is located in the promoter-proximal portion of the nusA operon [13].
  • However it has not been known whether the expression in E. coli of IF2 beta and IF2 gamma is dependent on or related to a posttranscriptional processing of the polycistronic nusA operon, containing infB, the gene for IF2 [14].

Anatomical context of nusA

  • This phenotype, previously seen in rpsL1204 strains whose ribosomes are pseudodependent on streptomycin and work at suboptimal elongation rate, indicates that RNA polymerase escapes from the ribosomes in the pyrE attenuator region in the nusA mutant [15].

Physical interactions of nusA


Other interactions of nusA

  • Thus, strains carrying either a nusA mutation or a nusB mutation have defects in transcription of 23S rRNA [12].
  • A novel plasmid vector, pAMH70 carrying both the lamB and nusA genes of Escherichia coli K12 was constructed [17].
  • The P35 gene, which is located in the nusA-infB operon in E. coll, was designated orp (osmoprotectant regulator of PLC) [18].
  • Implications of the sequence homologies found among htpR, rpoD, and nusA proteins are discussed [19].
  • Enzyme production was not further enhanced by any of the following chromosomal mutations: dnaA, recBC, tob, or nusA snu [20].

Analytical, diagnostic and therapeutic context of nusA


  1. The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli. Ishii, S., Ihara, M., Maekawa, T., Nakamura, Y., Uchida, H., Imamoto, F. Nucleic Acids Res. (1984) [Pubmed]
  2. Termination of transcription by nusA gene protein of Escherichia coli. Greenblatt, J., McLimont, M., Hanly, S. Nature (1981) [Pubmed]
  3. Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins. Drummond, M., Whitty, P., Wootton, J. EMBO J. (1986) [Pubmed]
  4. Isolation of a recombinant lambda phage carrying nusA and surrounding region of the Escherichia coli K-12 chromosome. Holowachuk, E.W., Friesen, J.D. Mol. Gen. Genet. (1982) [Pubmed]
  5. A plasmid to visualize and assay termination and antitermination of transcription in Escherichia coli. Franklin, N.C. Plasmid (1989) [Pubmed]
  6. Pausing and attenuation of in vitro transcription in the rrnB operon of E. coli. Kingston, R.E., Chamberlin, M.J. Cell (1981) [Pubmed]
  7. In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins. Nakamura, Y., Mizusawa, S. EMBO J. (1985) [Pubmed]
  8. Genetic interaction between the beta' subunit of RNA polymerase and the arginine-rich domain of Escherichia coli nusA protein. Ito, K., Egawa, K., Nakamura, Y. J. Bacteriol. (1991) [Pubmed]
  9. In vitro stimulation of Escherichia coli RNA polymerase sigma subunit synthesis by NusA protein. Peacock, S., Lupski, J.R., Godson, G.N., Weissbach, H. Gene (1985) [Pubmed]
  10. Involvement of the nusB gene products in transcription of Escherichia coli tryptophan operon in vitro. Kuroki, K., Ishii, S., Kano, Y., Miyashita, T., Nishi, K., Imamoto, F. Mol. Gen. Genet. (1982) [Pubmed]
  11. Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA). Plumbridge, J.A., Springer, M. J. Mol. Biol. (1983) [Pubmed]
  12. Transcriptional polarity in rRNA operons of Escherichia coli nusA and nusB mutant strains. Quan, S., Zhang, N., French, S., Squires, C.L. J. Bacteriol. (2005) [Pubmed]
  13. Differential transcriptional control of the two tRNA(fMet) genes of Escherichia coli K-12. Nagase, T., Ishii, S., Imamoto, F. Gene (1988) [Pubmed]
  14. Improved recombinant tandem expression of translation initiation factor IF2 in RNASE E deficient E. coli cells. Mortensen, K.K., Hajnsdorf, E., Regnier, P., Sperling-Petersen, H.U. Biochem. Biophys. Res. Commun. (1995) [Pubmed]
  15. Role of transcription pausing in the control of the pyrE attenuator in Escherichia coli. Andersen, J.T., Jensen, K.F., Poulsen, P. Mol. Microbiol. (1991) [Pubmed]
  16. Binding of rho factor to Escherichia coli RNA polymerase mediated by nusA protein. Schmidt, M.C., Chamberlin, M.J. J. Biol. Chem. (1984) [Pubmed]
  17. Use of lambda vehicles to isolate ompC-lacZ gene fusions in Salmonella typhimurium LT2. Harkki, A., Karkku, H., Palva, E.T. Mol. Gen. Genet. (1987) [Pubmed]
  18. Molecular characterization of mutants affected in the osmoprotectant-dependent induction of phospholipase C in Pseudomonas aeruginosa PAO1. Sage, A.E., Vasil, A.I., Vasil, M.L. Mol. Microbiol. (1997) [Pubmed]
  19. Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature. Yura, T., Tobe, T., Ito, K., Osawa, T. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  20. Cloning of the exonuclease III gene of Escherichia coli. Rogers, S.G., Weiss, B. Gene (1980) [Pubmed]
  21. Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes. Rich, J.J., Willis, D.K. J. Bacteriol. (1997) [Pubmed]
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