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Gene Review:

ORF2a' - minor glycoprotein

Simian hemorrhagic fever virus

Oleksiewicz, M.B. et al., Meulenberg, J.J. et al., Wu, W.H. et al., de Vries, A.A. et al., Nawagitgul, P. et al., et al.

Snijder, van Tol, Pedersen, Raamsman, de Vries, Meulenberg, Petersen-den Besten,

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Disease relevance of orf2

A heptad motif, VSRRIYQ, which is present in a single copy in ORF2 and 3 proteins, was identified; this arrangement is completely conserved in all European-type PRRSV sequences available [1].
These results suggest that the 2b protein is virion associated and the principal product of ORF2 [2].
The predicted LDV-P proteins, however, differed from those predicted for the LDV-C proteins between 25% for the ORF 2 protein and 1% for the ORF 7 nucleocapsid protein [3].

High impact information on orf2

The 3' part of EAV ORF 2a overlaps with the 5' part of the former ORF 2 (now renamed ORF 2b), which encodes the GS glycoprotein [4].
Using monospecific antisera as well as an antivirion serum, and by expression of individual ORFs, the genes for the structural proteins were identified: ORF 7 codes for N, ORF 6 for M, ORF 5 for GL, and ORF 2 for GS [5].
Intriguingly, a mutation associated with virulence/attenuation of an American vaccine strain of PRRSV also localized to this ORF2 protein segment and affected the hydrophobic face of the predicted amphipathic helix [1].
Nucleotide sequence analysis of North American PRRSV isolate SDSU-23983 revealed a small ORF within ORF2, named ORF2b, which, when translated, produced a 73-amino-acid nonglycosylated protein [2].
The protein has an apparent molecular weight of 29 to 30 kDa, was recognized by an ORF2-specific antipeptide serum in Western immunoblotting using sucrose gradient purified LV virions, and was shown to be N-glycosylated [6].

Chemical compound and disease context of orf2

Identification and characterization of a sixth structural protein of Lelystad virus: the glycoprotein GP2 encoded by ORF2 is incorporated in virus particles [6].

Other interactions of orf2

The ORFs 2, 3 and 4 overlapped each other and encoded polypeptides with predicted M(r) of 29.5 kDa (ORF 2), 28.7 kDa (ORF 3) and 19.5 kDa (ORF 4), respectively [7].

Analytical, diagnostic and therapeutic context of orf2

PCV2 and ORF2 ELISA detected antibodies to PCV2 and the capsid protein, respectively, in sera from pigs experimentally infected with PCV2 as early as 14 and 21 days postinoculation (dpi) [8].

References

Porcine B-cells recognize epitopes that are conserved between the structural proteins of American- and European-type porcine reproductive and respiratory syndrome virus. Oleksiewicz, M.B., Bøtner, A., Normann, P. J. Gen. Virol. (2002) [Pubmed]
A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Wu, W.H., Fang, Y., Farwell, R., Steffen-Bien, M., Rowland, R.R., Christopher-Hennings, J., Nelson, E.A. Virology (2001) [Pubmed]
Sequence of the genome of lactate dehydrogenase-elevating virus: heterogenicity between strains P and C. Palmer, G.A., Kuo, L., Chen, Z., Faaberg, K.S., Plagemann, P.G. Virology (1995) [Pubmed]
Identification of a novel structural protein of arteriviruses. Snijder, E.J., van Tol, H., Pedersen, K.W., Raamsman, M.J., de Vries, A.A. J. Virol. (1999) [Pubmed]
Structural proteins of equine arteritis virus. de Vries, A.A., Chirnside, E.D., Horzinek, M.C., Rottier, P.J. J. Virol. (1992) [Pubmed]
Identification and characterization of a sixth structural protein of Lelystad virus: the glycoprotein GP2 encoded by ORF2 is incorporated in virus particles. Meulenberg, J.J., Petersen-den Besten, A. Virology (1996) [Pubmed]
Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus. Morozov, I., Meng, X.J., Paul, P.S. Arch. Virol. (1995) [Pubmed]
Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Nawagitgul, P., Harms, P.A., Morozov, I., Thacker, B.J., Sorden, S.D., Lekcharoensuk, C., Paul, P.S. Clin. Diagn. Lab. Immunol. (2002) [Pubmed]

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