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Gene Review

ORF2a  -  minor glycoprotein

Simian hemorrhagic fever virus

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Disease relevance of SHFVgp05

  • Posttranslational processing and identification of a neutralization domain of the GP4 protein encoded by ORF4 of Lelystad virus [1].
  • This hitherto unrecognized immunodominance likely caused phages displaying the ORF4 ES to outcompete phages displaying other ES during library screening with porcine sera and accounted for our failure to identify more than two ES in the structural genes of PRRSV [2].
  • A region of the PRRS virus genome containing portions of ORF4 and ORF5 was amplified directly from tissues of infected pigs from birth to 132 days of age [3].
  • To investigate the nature of the PRRSV ORFs 7, 5 and 4 products, we have cloned the envelope (ORF5) nucleocapsid (ORF7) and ns4 (ORF4) protein genes in the bacterial expression vector pMAL(TM)-c2 under control of the "tac" promoter and expressed the proteins in E. coli [4].

High impact information on SHFVgp05

  • To map the binding site of the MAbs, chimeric constructs composed of ORF4 of LV and VR2332 were generated [1].
  • Through the use of overlapping reading frames, the same PRRSV genetic locus codes for the ORF 3 "RKASLSTS" sequence, and a previously described ORF 4 epitope (Meulenberg, J. J. M., Van Nieuwstadt, A. P., Van Essen-Zandbergen, A., and Langeveld, J. P. M., 1997, J. Virol. 71, 6061-6067) [5].
  • The RT-PCR RFLP typing assay was performed using 2 different sets of primers, amplifying 716 or 933 base pairs of ORF 4, 5, and 6 of PRRSV [6].
  • The ORFs 2, 3 and 4 overlapped each other and encoded polypeptides with predicted M(r) of 29.5 kDa (ORF 2), 28.7 kDa (ORF 3) and 19.5 kDa (ORF 4), respectively [7].
  • Comparison of the ORF 4 sequences of VR2385 with that of another U.S. isolate MN-1b revealed only 86% amino acid sequence homology and the presence of deletions in the ORF 4 of MN-1b [7].

Chemical compound and disease context of SHFVgp05


Other interactions of SHFVgp05

  • The results also indicate that neutralization epitopes for PRRS virus are present on the viral envelope glycoproteins encoded by ORF 4 and ORF 5 [9].

Analytical, diagnostic and therapeutic context of SHFVgp05

  • In some animals, sera diluted 1:62,500 still gave weak positive enzyme immunoassay reactivity against the ORF4 ES [2].
  • In contrast, Porcilis-vaccinated animals seroconverted in the ORF4 peptide ELISA at 21 days post-vaccination [8].
  • The cDNA clones containing PRRSV specific sequences were selected using a VR2385 ORF 4 specific PCR probe and sequenced [7].


  1. Posttranslational processing and identification of a neutralization domain of the GP4 protein encoded by ORF4 of Lelystad virus. Meulenberg, J.J., van Nieuwstadt, A.P., van Essen-Zandbergen, A., Langeveld, J.P. J. Virol. (1997) [Pubmed]
  2. Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes. Oleksiewicz, M.B., Bøtner, A., Toft, P., Normann, P., Storgaard, T. J. Virol. (2001) [Pubmed]
  3. The evolution of porcine reproductive and respiratory syndrome virus: quasispecies and emergence of a virus subpopulation during infection of pigs with VR-2332. Rowland, R.R., Steffen, M., Ackerman, T., Benfield, D.A. Virology (1999) [Pubmed]
  4. Identification and characterization of the porcine reproductive and respiratory virus ORFs 7, 5 and 4 products. Mounir, S., Mardassi, H., Dea, S. Adv. Exp. Med. Biol. (1995) [Pubmed]
  5. Emergence of porcine reproductive and respiratory syndrome virus deletion mutants: correlation with the porcine antibody response to a hypervariable site in the ORF 3 structural glycoprotein. Oleksiewicz, M.B., Bøtner, A., Toft, P., Grubbe, T., Nielsen, J., Kamstrup, S., Storgaard, T. Virology (2000) [Pubmed]
  6. Restriction fragment length polymorphism of porcine reproductive and respiratory syndrome viruses recovered from Ontario farms, 1998-2000. Cai, H.Y., Alexander, H., Carman, S., Lloyd, D., Josephson, G., Maxie, M.G. J. Vet. Diagn. Invest. (2002) [Pubmed]
  7. Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus. Morozov, I., Meng, X.J., Paul, P.S. Arch. Virol. (1995) [Pubmed]
  8. Discriminating between serological responses to European-genotype live vaccine and European-genotype field strains of porcine reproductive and respiratory syndrome virus (PRRSV) by peptide ELISA. Oleksiewicz, M.B., Stadejek, T., Maćkiewicz, Z., Porowski, M., Pejsak, Z. J. Virol. Methods (2005) [Pubmed]
  9. Antibody and cellular immune responses of swine following immunisation with plasmid DNA encoding the PRRS virus ORF's 4, 5, 6 and 7. Kwang, J., Zuckermann, F., Ross, G., Yang, S., Osorio, F., Liu, W., Low, S. Res. Vet. Sci. (1999) [Pubmed]
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