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Gene Review

ORF4'  -  minor glycoprotein

Simian hemorrhagic fever virus

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Disease relevance of orf3


High impact information on orf3

  • Through the use of overlapping reading frames, the same PRRSV genetic locus codes for the ORF 3 "RKASLSTS" sequence, and a previously described ORF 4 epitope (Meulenberg, J. J. M., Van Nieuwstadt, A. P., Van Essen-Zandbergen, A., and Langeveld, J. P. M., 1997, J. Virol. 71, 6061-6067) [1].
  • The nucleic acid homology with corresponding ORFs of the European PRRSV isolate Lelystad virus (LV) was 65% for ORF 2, 64% for ORF 3 and 66% for ORF 4 [5].

Biological context of orf3


Analytical, diagnostic and therapeutic context of orf3

  • Together, our results show that ORF3 sequencing is a valuable epidemiologic tool for examining the emergence and spread of PRRSV in Europe. As such, the panel of well-characterized and highly divergent ORF3 sequences described in this study provides a reference point for future molecular epidemiologic studies [6].
  • The ORF3 and ORF7 regions of the individual viruses were amplified by the polymerase chain reaction (PCR) and their sequences were determined using internal sense and antisense primers [7].
  • Tested convalescent pig sera that were found to be seropositive to PRRSV by indirect immunofluorescence reacted positively with the recombinant GST-ORF3 fusion protein by immunoblotting [4].
  • Based on the size of the ORF 3, 3-1 and 4 RT/PCR products, TGEV and PRCV strains could be quickly and easily differentiated into three groups designated TGEV Miller, Purdue types and PRCV [8].


  1. Emergence of porcine reproductive and respiratory syndrome virus deletion mutants: correlation with the porcine antibody response to a hypervariable site in the ORF 3 structural glycoprotein. Oleksiewicz, M.B., Bøtner, A., Toft, P., Grubbe, T., Nielsen, J., Kamstrup, S., Storgaard, T. Virology (2000) [Pubmed]
  2. Production, characterization and reactivity of monoclonal antibodies to porcine reproductive and respiratory syndrome virus. Drew, T.W., Meulenberg, J.J., Sands, J.J., Paton, D.J. J. Gen. Virol. (1995) [Pubmed]
  3. Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) are encoded by the carboxyterminal portion of viral open reading frame 3. Katz, J.B., Shafer, A.L., Eernisse, K.A., Landgraf, J.G., Nelson, E.A. Vet. Microbiol. (1995) [Pubmed]
  4. A nonstructural and antigenic glycoprotein is encoded by ORF3 of the IAF-Klop strain of porcine reproductive and respiratory syndrome virus. Gonin, P., Mardassi, H., Gagnon, C.A., Massie, B., Dea, S. Arch. Virol. (1998) [Pubmed]
  5. Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus. Morozov, I., Meng, X.J., Paul, P.S. Arch. Virol. (1995) [Pubmed]
  6. A molecular clock dates the common ancestor of European-type porcine reproductive and respiratory syndrome virus at more than 10 years before the emergence of disease. Forsberg, R., Oleksiewicz, M.B., Petersen, A.M., Hein, J., Bøtner, A., Storgaard, T. Virology (2001) [Pubmed]
  7. Variation in open reading frames 3, 4 and 7 among porcine reproductive and respiratory syndrome virus isolates in the UK. Drew, T.W., Lowings, J.P., Yapp, F. Vet. Microbiol. (1997) [Pubmed]
  8. Field isolates of transmissible gastroenteritis virus differ at the molecular level from the Miller and Purdue virulent and attenuated strains and from porcine respiratory coronaviruses. Kwon, H.M., Saif, L.J., Jackwood, D.J. J. Vet. Med. Sci. (1998) [Pubmed]
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