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Gene Review:

CD8B - CD8b molecule

Homo sapiens

Synonyms: CD8B1, LEU2, LY3, LYT3, P37, ...

Bilic, I. et al., Rumpold, H. et al., Kieffer, L.J. et al., Senju, M. et al., Cooper, K.D. et al., et al.

Kioussis, Ellmeier,

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High impact information on CD8B

Negative regulation of CD8 expression via Cd8 enhancer-mediated recruitment of the zinc finger protein MAZR [1].
Deletion of specific Cd8 enhancers leads to variegated expression of CD8alphabeta heterodimers in double-positive thymocytes [1].
The zinc finger protein MAZR bound the Cd8 enhancer and interacted with the nuclear receptor corepressor N-CoR complex in double-negative thymocytes [1].
Here we show CD8 variegation is correlated with an epigenetic 'off' state, linking Cd8 enhancer function with chromatin remodeling of the adjacent genes Cd8a and Cd8b1 (Cd8) [1].
The T-lymphocyte-specific reference gene CD3E remained stable while CD8B was upregulated 2.3-fold [2].

Biological context of CD8B

Chromatin and CD4, CD8A and CD8B gene expression during thymic differentiation [3].
Phylogeny and structure of the Ly-2 and Ly-3 (CD8) complex [4].
Leu-3/Leu-2 (CD4/CD8) ratio of lamina propria lymphocytes (LPL) of CD (mean +/- SD: 1.9 +/- 0.8, P less than 0.01) was significantly decreased compared with controls (3.3 +/- 1.1), because of an increased number of CD8+ lymphocytes [5].
Again, phenotypes similar to those observed on LGL enriched by Percoll gradient centrifugation were found: of VEP13+ cells 84 +/- 6% reacted with OKM1, 82 +/- 5% with OKT10, 52 +/- 17% with anti-human Lyt3, and 48 +/- 14% with anti-Leu2a, whereas VEP8, VEP9, VIM-D5, VIB-C5, T6, Leu1, Leu3a antigens were not expressed on VEP13+ cells [6].

Anatomical context of CD8B

To locate elements regulating the human CD8 gene complex, we mapped nuclear matrix attachment regions (MARs) and DNase I hypersensitive (HS) sites over a 100-kb region that included the CD8B gene, the intergenic region, and the CD8A gene [7].
In the same way, Lyt3 was used for determination of the total T lymphocytes [8].
Unfractionated mononuclear leukocytes (MNL) were sensitized with appropriate dilutions of monoclonal antibodies to human Lyt3 , OKT4 and OKT8 antigens, and to a monocyte-myeloid line antigen [9].

Analytical, diagnostic and therapeutic context of CD8B

In patients with Stage IIIb disease, there were trends toward higher recurrence, lower survival, or disease free survival rates in the Ly2 + Ly3 groups compared with the Ly1 group (P < 0.1) [10].

References

Negative regulation of CD8 expression via Cd8 enhancer-mediated recruitment of the zinc finger protein MAZR. Bilic, I., Koesters, C., Unger, B., Sekimata, M., Hertweck, A., Maschek, R., Wilson, C.B., Ellmeier, W. Nat. Immunol. (2006) [Pubmed]
Commonly Used Reference Genes are Actively Regulated in In vitro Stimulated Lymphocytes. Røge, R., Thorsen, J., Tørring, C., Ozbay, A., Møller, B.K., Carstens, J. Scand. J. Immunol. (2007) [Pubmed]
Chromatin and CD4, CD8A and CD8B gene expression during thymic differentiation. Kioussis, D., Ellmeier, W. Nat. Rev. Immunol. (2002) [Pubmed]
Phylogeny and structure of the Ly-2 and Ly-3 (CD8) complex. Gillespie, M.T., Panaccio, M., McKenzie, I.F., Deacon, N.J. Immunol. Cell Biol. (1989) [Pubmed]
Two-color immunofluorescence and flow cytometric analysis of lamina propria lymphocyte subsets in ulcerative colitis and Crohn's disease. Senju, M., Wu, K.C., Mahida, Y.R., Jewell, D.P. Dig. Dis. Sci. (1991) [Pubmed]
Phenotypes of human large granular lymphocytes as defined by monoclonal antibodies. Rumpold, H., Kraft, D., Obexer, G., Radaszkiewicz, T., Majdic, O., Bettelheim, P., Knapp, W., Böck, G. Immunobiology (1983) [Pubmed]
Identification of a candidate regulatory region in the human CD8 gene complex by colocalization of DNase I hypersensitive sites and matrix attachment regions which bind SATB1 and GATA-3. Kieffer, L.J., Greally, J.M., Landres, I., Nag, S., Nakajima, Y., Kohwi-Shigematsu, T., Kavathas, P.B. J. Immunol. (2002) [Pubmed]
Evaluation of the circulating and splenic lymphocyte subpopulations in patients with non-Hodgkin lymphomas and Hodgkin's disease using monoclonal antibodies. Herrmann, F., Sieber, G., Jauer, B., Lochner, A., Komischke, B., Rühl, H. Blut (1983) [Pubmed]
Direct depletion and purification of monoclonal antibody defined cells from unfractionated human mononuclear leukocytes using antibody coated polystyrene Petri dishes. Cooper, K.D., Kang, K., Bush, T.L., Hanifin, J.M. Journal of clinical & laboratory immunology. (1984) [Pubmed]
Carcinomatous lymphatic permeation. Prognostic significance in patients with rectal carcinoma--a long term prospective study. Shirouzu, K., Isomoto, H., Morodomi, T., Kakegawa, T. Cancer (1995) [Pubmed]

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