High impact information on Cd8b1

• A mutant mouse strain without CD8 (Lyt-2 and Lyt-3) expression on the cell surface has been generated by disrupting the Lyt-2 gene using embryonic stem cell technology [1].
• These results suggested that the Lyt-2, Lyt-3 macromolecules are associated with but do not serve as the T cell antigen receptor [2].
• A heterodimer composed of the human Lyt-2 and Lyt-3 molecules may have importance in T cell-target cell interactions [3].
• The nucleotide and predicted amino acid sequence of the murine Ly-3 cDNA, isolated by using the rat Ly-3 cDNA clone pX9.15, together with the amino acid sequence of Ly-3.1 peptides and the N terminus, are presented here [4].
• Searches of protein data bases revealed that Ly-3 is a member of the immunoglobulin superfamily with significant homology to Ly-2, immunoglobulin variable region kappa and lambda light chains, and the beta chain of the T-cell receptor [4].

Biological context of Cd8b1

• The alignment of peptide data from the Ly-3.1 antigen with that of the predicted amino acid sequence of the Ly-3.2 antigen confirmed that the putative Ly-3 cDNA clones do in fact encode the Ly-3 protein [4].
• We have isolated and analyzed cDNA and cosmid clones corresponding to the Ly-3 subunit [5].
• We have determined the complete primary structure of Lyt-3 from the nucleotide sequence of its cDNA clones [6].
• Isolation and characterization of the mouse CD8 beta-chain (Ly-3) genes. Absence of an intervening sequence between V- and J-like gene segments [7].
• Recombination has been detected for the first time between chromosome 6 loci controlling kappa chain expression in normal mouse serum immunoglobulin and the Lyt-3 locus [8].

Anatomical context of Cd8b1

• Thus Lyt-3 is not required structurally for the spontaneous expression of Lyt-2 on lymphoid cells [9].
• Staining with monoclonal and polyclonal antibodies to the CD8 alpha (Ly-2) and CD8 beta (Ly-3) subunits indicates that only CD8 alpha is expressed by these activated TcR gamma/delta cells [10].
• The early restitution of the thymus of bone marrow chimeras was investigated by the immunoperoxidase technique using monoclonal antibodies against Thy-1 and Lyt-1, Lyt-2, Lyt-3 [11].
• All Lyt-2-positive hybrids expressed functional surface Lyt-3 encoded by the CTL fusion partner, demonstrating that expression of the Lyt-3 gene is not sensitive to the negative regulation which shuts off the endogenous Lyt-2 gene in hybrids of class I-specific CTLs with the 3B2 or BW5147 cell lines [12].

Associations of Cd8b1 with chemical compounds

• Molecular cloning of Lyt-3, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to immunoglobulin and T-cell receptor variable and joining regions [6].
• Analysis of the predicted amino acid sequence indicates that the Lyt-3 polypeptide has a 21-amino acid leader peptide, and the mature protein consists of an NH2-terminal region of 146 amino acids, a transmembrane region of 27 residues, and a C-terminal region of 19 amino acids [6].
• Lyt-2 and Lyt-3 alloantigens: precipitation with monoclonal and conventional antibodies and analysis on one- and two-dimensional polyacrylamide gels [13].
• Treatment of cells with either sodium azide or 2-mercaptoethanol followed by N-ethyl maleimide to reduce and block sulfhydryl groups inhibited the patch formation caused by simultaneous incubation with both Lyt-2 and Lyt-3 antibodies [14].

Regulatory relationships of Cd8b1

• Thus the Lyt-3 gene is not subject to the same negative regulatory influences as the Lyt-2 gene [15].
• Lyt-3 is a membrane glycoprotein expressed on thymocytes and class I major histocompatibility complex-restricted cytotoxic T cells [6].

Other interactions of Cd8b1

• In the mouse, CD8 is a heterodimer composed of Ly-2 and Ly-3 chains [5].
• On the basis of these data, the Rn7s-6 sequence may be placed within 1.3 centimorgans of Ly-3 and one of the Igk-V-region markers, Igk-Ef1 [16].
• Using monoclonal antibodies and multiparameter fluorescence analyses, we show that the expression of Lyt-1, Lyt-2, and Lyt-3 on T cell subpopulations is more complex than was originally recognized by the cytotoxic depletion studies with conventional reagents that defined the Lyt-1+2+3+, Lyt-1+2-3-, and Lyt-1-2+3+ populations [17].
• Pretreatment of immune spleen cells with Thy-1, Lyt-2, or Lyt-3 antisera and complement eliminated cytotoxic activity, indicating that effector cells for RL male 1 lysis are T cells [18].
• The C.C58 and C.AKR congeneic strains of mice differ from BALB/c at loci on chromosome 6 which govern kappa light chain variable region (V kappa) polymorphisms and the Lyt-2 and Lyt-3 alloantigens [19].

Analytical, diagnostic and therapeutic context of Cd8b1

• Although two alleles of Ly-3 have been previously defined serologically, Northern blot analyses of Ly-3 mRNA from a series of inbred mouse strains show unusual polymorphisms in the lengths of Ly-3 mRNA species defining at least three allelic variants of this gene [20].
• Southern hybridization and structural analysis of DNA fragments generated using the polymerase chain reaction demonstrated that hybrids contain several species of Lyt-3 RNA, one of which lacks the exon encoding the extracellular V-like domain and appears to be the product of an alternatively-spliced RNA transcript [15].
• The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization [21].
• Specific precipitates obtained using anti-Ly-2.1 serum yielded SDS-PAGE profiles identical to that obtained with anti-Ly-3.1 serum, suggesting that the Ly-2 and Ly-3 antigens have the same molecular weight distribution [21].

References