Disease relevance of CPA5

• Mouse pro-CPA5 protein expressed in Sf9 cells using the baculovirus system was retained in the particulate fraction of the cells and was not secreted into the media [1].
• Bipartite geminiviruses infecting tomatoes in Thailand were detected by polymerase chain reaction (PCR) using CPA5/CPA2 primers [2].

High impact information on CPA5

• Pro-CPA5 was not enzymatically active toward standard CPA substrates, but after incubation with prohormone convertase 4 the resulting protein was able to cleave furylacryloyl-Gly-Leu, with 3-4-fold greater activity at pH 7.4 than at 5 [1].
• In situ hybridization analysis shows that CPA5 is localized to testis germ cells [1].
• The deduced amino acid sequence of human CPA5 has highest amino acid sequence identity (60%) to CPA1 [1].
• Therefore, saturated phosphatidylcholine (Sat PC) was measured to quantify the surfactant necessary for preterm lambs to breathe successfully on a CPAP of 5 cm H(2)O (CPAP 5) [3].
• The imprinting status of two (TSGA13 and CPA5) was not conclusive, because of their weak and/or tissue-specific expression and inconstant results [4].

Biological context of CPA5

• The active site of CPA5 is predicted to cleave substrates with C-terminal hydrophobic residues, as do CPA1, -2, and -3 [1].

Anatomical context of CPA5

• The cDNA encoding a mouse homolog of human CPA5 was isolated from a testis library and sequenced [1].
• In the AtT-20 corticotroph cell line, CPA-5 has a perinuclear distribution [5].

Analytical, diagnostic and therapeutic context of CPA5

• Using Northern blot analysis, CPA5 mRNA is detected in testis but not in kidney, liver, brain, or lung [1].
• Physiologic responses of other 132-day preterm lambs after 2 or 6 hours of CPAP 5, 8 cm H(2)O CPAP (CPAP 8), or mechanical ventilation were then characterized [3].
• To test whether CPA-5 protein has a distribution pattern in pituitary that is consistent with a role for this enzyme in the non-basic processing of proopiomelanocortin-derived peptides such as beta-endorphin and adrenocorticotropin, we examined the distribution of CPA-5 using immunocytochemistry [5].

References