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Gene Review

AURAVgp1  -  Polyprotein 1

Aura virus

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Disease relevance of AURAVgp1

  • The Semliki Forest virus capsid protein contains a chymotrypsin-like protease domain that must fold before it can autocatalytically cleave the protein from a larger polyprotein precursor [1].
  • Here we describe a new packaging system for Semliki Forest virus (SFV) based on a the use of a two-helper system in which the capsid and spike proteins of the C-p62-6K-E1 polyprotein are expressed from two independent RNA molecules [2].
  • The polyprotein P1234 is produced when translational readthrough occurs or when the opal termination codon has been replaced by a sense codon in the alphavirus genome [3].
  • The structural proteins are also translated as a polyprotein precursor which is cleaved to produce a nucleocapsid protein and two integral membrane glycoproteins as well as two small peptides not present in the mature virion [4].
  • A high level of similarity was detected between the leader protease of foot-and-mouth-disease virus and the protease of murine hepatitis coronavirus which cleaves the N-terminal p28 protein from the polyprotein [5].

High impact information on AURAVgp1

  • In addition, the capsid protein has an autoproteolytic activity whereby the C-terminal tryptophan is used as the substrate for cotranslational cleavage of the viral structure polyprotein [6].
  • This construct has correct processing of the replicase polyprotein, produces viable virus and expresses detectable EGFP fluorescence upon infection of cultured cells and cells of the mouse brain [7].
  • At the C terminus of the C protein is a stretch of 20 hydrophobic aa which also serves as the signal sequence for E2, indicating that the cleavage of C from the polyprotein precursor may be catalyzed by signalase in the lumen of the endoplasmic reticulum [8].
  • Translation of the 26S mRNA began at the first AUG (positions 59 to 61) initiation codon and continued with an open reading frame that coded for a polyprotein of 1258 amino acids ending at a UAA ochre termination codon (positions 3776 to 3778) [9].
  • The deduced amino acid sequence of the VEE polyprotein shows an overall homology of 44 to 46% with the precursor polyproteins of SIN, SF, and RR viruses [10].

Associations of AURAVgp1 with chemical compounds

  • Negative strand synthesis is unstable because of the failure to form initial transcription complexes after host factors that are part of the replicase are depleted or the half-life of polyprotein precursors like P23 is shortened [11].


  1. Co-translational folding of an alphavirus capsid protein in the cytosol of living cells. Nicola, A.V., Chen, W., Helenius, A. Nat. Cell Biol. (1999) [Pubmed]
  2. Two-helper RNA system for production of recombinant Semliki forest virus particles. Smerdou, C., Liljeström, P. J. Virol. (1999) [Pubmed]
  3. Effects of an Opal Termination Codon Preceding the nsP4 Gene Sequence in the O'Nyong-Nyong Virus Genome on Anopheles gambiae Infectivity. Myles, K.M., Kelly, C.L., Ledermann, J.P., Powers, A.M. J. Virol. (2006) [Pubmed]
  4. Complete nucleotide sequence of the genomic RNA of Sindbis virus. Strauss, E.G., Rice, C.M., Strauss, J.H. Virology (1984) [Pubmed]
  5. Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses. Gorbalenya, A.E., Koonin, E.V., Lai, M.M. FEBS Lett. (1991) [Pubmed]
  6. Role of the C-terminal tryptophan residue for the structure-function of the alphavirus capsid protein. Skoging, U., Liljeström, P. J. Mol. Biol. (1998) [Pubmed]
  7. Insertion of EGFP into the replicase gene of Semliki Forest virus results in a novel, genetically stable marker virus. Tamberg, N., Lulla, V., Fragkoudis, R., Lulla, A., Fazakerley, J.K., Merits, A. J. Gen. Virol. (2007) [Pubmed]
  8. Sequence of the region coding for virion proteins C and E2 and the carboxy terminus of the nonstructural proteins of rubella virus: comparison with alphaviruses. Frey, T.K., Marr, L.D. Gene (1988) [Pubmed]
  9. Nucleotide sequence of the genome region encoding the 26S mRNA of eastern equine encephalomyelitis virus and the deduced amino acid sequence of the viral structural proteins. Chang, G.J., Trent, D.W. J. Gen. Virol. (1987) [Pubmed]
  10. Nucleotide sequence of the 26 S mRNA of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and deduced sequence of the encoded structural proteins. Kinney, R.M., Johnson, B.J., Brown, V.L., Trent, D.W. Virology (1986) [Pubmed]
  11. Alphavirus positive and negative strand RNA synthesis and the role of polyproteins in formation of viral replication complexes. Sawicki, D.L., Sawicki, S.G. Arch. Virol. Suppl. (1994) [Pubmed]
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