Disease relevance of fruR

• We have sequenced the fruR gene and flanking DNA fragments from Escherichia coli K12 and Salmonella typhimurium LT2 [1].
• Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2 [1].
• Significant nucleotide identity (52.1%) was seen between hpm and the shl hemolysin gene sequences of Serratia marcescens despite differences in the G+C contents of these genes (hpm, 38%; shl, 65%) [2].
• The amino acid sequence encoded by the ORF contained a helix-turn-helix DNA-binding domain and was homologous to the catabolite control protein, CcpA, from Bacillus subtilis and Bacillus megaterium, a tRNA repressor of E. coli encoded by the shl gene, and the GalR, Lacl and PurR repressors of E. coli [3].

High impact information on fruR

• Consistent with the in vitro results, pts P0 transcription did not increase as much in cells grown in the presence of fructose or in fruR- mutant cells as in cells grown in the presence of glucose [4].
• DNA sequence analysis revealed a missense mutation in the cra gene, formerly known as fruR [5].
• Molecular cloning, nucleotide sequence, and expression of shl, a new gene in the 2-minute region of the genetic map of Escherichia coli [6].
• The amino acid sequence predicted from the nucleotide sequence of the shl gene indicated a similarity to the GalR and LacI repressor proteins, suggesting it is a transcription regulator [6].
• We found that a gene located at the 2-min region, designated shl, inhibited the growth of supH-harboring cells when carried by a high-copy-number plasmid, whereas the same plasmid had no visible effect when present in parental cells [6].

Biological context of fruR

• The fruR gene codes for a protein that represses the fru operon and activates the pps gene for PEP synthase [1].
• The sequence is identical to that of the E. coli shl gene (mnemonic for suppressor-H-linked phenotype) [1].

Associations of fruR with chemical compounds

• In this study we have demonstrated that cra mutant strains synthesize 10-fold less cAMP than isogenic wild-type strains, specifically when grown in fructose-containing minimal media [7].

References