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Gene Review

ndk  -  multifunctional nucleoside diphosphate...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2514, JW2502
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Disease relevance of ndk


High impact information on ndk

  • Mutants of E. coli lacking Ndk exhibit normal growth rates but show a mutator phenotype that cannot be entirely attributed to the absence of Ndk catalytic activity or to an imbalance in cellular triphosphates [5].
  • Escherichia coli nucleoside-diphosphate kinase (Ndk) catalyzes nucleoside triphosphate synthesis and maintains intracellular triphosphate pools [5].
  • It has been suggested previously that Ndk, similar to its human counterparts, possesses nuclease and DNA repair activities, including the excision of uracil from DNA, an activity normally associated with the Ung and Mug uracil-DNA glycosylases (UDGs) in E. coli [5].
  • Co-purification of Ung and Ndk through multicolumn low pressure and nickel-nitrilotriacetic acid affinity chromatography suggests that the interaction occurs in a cellular context, as was also suggested by co-immunoprecipitation of endogenous Ung and Ndk from cellular extracts [5].
  • However, ndk disruption unbalances the dNTP pools and stimulates mutagenesis [6].

Chemical compound and disease context of ndk

  • An E. coli strain carrying deletions in ndk and pyrA and pyrF, the structural genes for both pyruvate kinases, also grows and supports T4 infection [7].

Biological context of ndk

  • The ndk gene appears to be in a monocistronic operon and, when cloned in a pUC vector, NDP kinase was overproduced at a level of approx. 25% of total cellular proteins [8].
  • As shown elsewhere, these ndk mutant cells display both a mutator phenotype and deoxyribonucleotide pool abnormalities [7].
  • The finding that mutagenesis and pool imbalances are mitigated in both anaerobic and T4-infected cultures provides strong, if circumstantial, evidence that the mutator phenotype of ndk mutant cells is a result of the dNTP imbalance [7].
  • Characterization of a cytosolic nucleoside diphosphate kinase associated with cell division and growth in potato [9].

Associations of ndk with chemical compounds

  • AlgH, a putative transcriptional regulator, is involved in regulating alginate biosynthesis as well as nucleoside diphosphate kinase activity and succinyl coenzyme A synthetase activity in P. aeruginosa [10].


  1. Analysis of a Streptomyces coelicolor A3(2) locus containing the nucleoside diphosphate kinase (ndk) and folylpolyglutamate synthetase (folC) genes. Burger, A., Brandt, B., Süsstrunk, U., Thompson, C.J., Wohlleben, W. FEMS Microbiol. Lett. (1998) [Pubmed]
  2. Escherichia coli nucleoside diphosphate kinase interactions with T4 phage proteins of deoxyribonucleotide synthesis and possible regulatory functions. Shen, R., Olcott, M.C., Kim, J., Rajagopal, I., Mathews, C.K. J. Biol. Chem. (2004) [Pubmed]
  3. Nucleoside diphosphate kinase from Escherichia coli. Almaula, N., Lu, Q., Delgado, J., Belkin, S., Inouye, M. J. Bacteriol. (1995) [Pubmed]
  4. Nucleoside diphosphate kinase from Myxococcus xanthus. II. Biochemical characterization. Muñoz-Dorado, J., Inouye, S., Inouye, M. J. Biol. Chem. (1990) [Pubmed]
  5. Molecular and Functional Interactions between Escherichia coli Nucleoside-diphosphate Kinase and the Uracil-DNA Glycosylase Ung. Goswami, S.C., Yoon, J.H., Abramczyk, B.M., Pfeifer, G.P., Postel, E.H. J. Biol. Chem. (2006) [Pubmed]
  6. Molecular interactions involving Escherichia coli nucleoside diphosphate kinase. Shen, R., Wheeler, L.J., Mathews, C.K. J. Bioenerg. Biomembr. (2006) [Pubmed]
  7. Effects of T4 phage infection and anaerobiosis upon nucleotide pools and mutagenesis in nucleoside diphosphokinase-defective Escherichia coli strains. Zhang, X., Lu, Q., Inouye, M., Mathews, C.K. J. Bacteriol. (1996) [Pubmed]
  8. Nucleoside diphosphate kinase from Escherichia coli; its overproduction and sequence comparison with eukaryotic enzymes. Hama, H., Almaula, N., Lerner, C.G., Inouye, S., Inouye, M. Gene (1991) [Pubmed]
  9. Characterization of a cytosolic nucleoside diphosphate kinase associated with cell division and growth in potato. Dorion, S., Matton, D.P., Rivoal, J. Planta (2006) [Pubmed]
  10. Cloning, high yield overexpression, purification, and characterization of AlgH, a regulator of alginate biosynthesis in Pseudomonas aeruginosa. Bieber Urbauer, R.J., Gilmore, J.M., Rosasco, S.E., Hattle, J.M., Cowley, A.B., Urbauer, J.L. Protein Expr. Purif. (2005) [Pubmed]
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