Disease relevance of cheY

• Identification of a chemotaxis operon with two cheY genes in Rhodobacter sphaeroides [1].
• In this study, we found a fourth cheY and expressed these R. sphaeroides proteins in E. coli [2].
• Characterization of the cheY genes from Leptospira interrogans and their effects on the behavior of Escherichia coli [3].

Psychiatry related information on cheY

• The unusual behaviour of the cheA mutant and the identification of two homologues of cheY suggests that R. sphaeroides has at least two pathways controlling motor activity [1].

High impact information on cheY

• Single crystals of the 14.1-kDa cheY gene product from Escherichia coli have been grown from buffered ammonium sulfate solutions using the combined methods of microdialysis and pulsed diffusion [4].
• We found that chemotaxis in the cheW Che+ suppressor depended on both cheY and cheA [5].
• We deleted the cheY genes individually or in combination and found that only the cheY3 deletion impaired chemotaxis, reinforcing the previous conclusion that che cluster II is involved in chemotaxis [6].
• To investigate the contribution of Thr87 to signaling, we characterized, genetically and biochemically, several cheY mutants with amino acid substitutions at this position [7].
• We have generated a class of cheY mutations selected for dominant suppression of fliG mutations [8].

Biological context of cheY

• DNA sequences downstream of the cheY and cheZ genes were able to complement PC3 but not PC4 [9].
• The resulting 5,251-bp sequence contained five open reading frames, including cheA, cheW, and cheY [10].
• To express this synthetic gene in Escherichia coli, an expression vector, pJC264, was constructed by inserting portions of the E. coli cheB and cheY gene complex into the plasmid pUC13 [11].

Regulatory relationships of cheY

• Genetic analysis of Escherichia coli has identified fliG and fliM as genes in which mutations occur that allele specifically suppress cheY mutations, indicating interactions among these gene products [8].

References