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Gene Review

mtlA  -  mannitol-specific PTS enzyme: IIA, IIB and...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3588, JW3573, mtlC
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Disease relevance of mtlA


High impact information on mtlA

  • The nucleotide sequence of the mtlA gene, which codes for the mannitol-specific Enzyme II of the Escherichia coli phosphotransferase system, is presented [4].
  • Repetitive extragenic palindromic (REP) sequences, forming stem-loop structures in the intercistronic region between mtlA and mtlD and following the mtlD gene were identified [5].
  • Cell lysis in response to indole acrylic acid, which causes the lethal overexpression of the plasmid-encoded mtlA gene, was also scored [6].
  • A 2-kilobase pair fragment of the mtl operon DNA containing the mtlA gene was subcloned into plasmid pACYC184 [7].
  • Localization of the mtlA gene on the cloned mtl operon DNA allowed in vitro construction of plasmid derivatives containing specific deletions in the mtl region [7].

Chemical compound and disease context of mtlA


Biological context of mtlA

  • The gene order in this operon, induced by D-mannitol, is mtlC A D [8].
  • Several mtlA-phoA gene fusions encoding fused proteins with N-terminal regions derived from the mannitol permease and C-terminal regions derived from the mature portion of alkaline phosphatase were constructed [9].
  • Among the 17 putative ORFs identified, orf1 and orf2 seem to correspond to mtlA and mtlB, encoding mannitol-specific phosphotransferase enzyme II and mannitol-1-phosphate dehydrogenase, respectively. orf4 seems to be another signal peptidase I gene (sipS) of B. subtilis [10].

Analytical, diagnostic and therapeutic context of mtlA

  • Sequence analysis of deduced protein products indicated that the mtlA and mtlF genes respectively encode the IICB and IIA components of the mannitol PTS, which is a member of the fructose-mannitol (Fru) family [11].


  1. The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026. Otte, S., Lengeler, J.W. FEMS Microbiol. Lett. (2001) [Pubmed]
  2. Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions. Sugiyama, J.E., Mahmoodian, S., Jacobson, G.R. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  3. The mannitol-specific enzyme II (mtlA) gene and the mtlR gene of the PTS of Streptococcus mutans. Honeyman, A.L., Curtiss, R. Microbiology (Reading, Engl.) (2000) [Pubmed]
  4. Mannitol-specific enzyme II of the bacterial phosphotransferase system. III. The nucleotide sequence of the permease gene. Lee, C.A., Saier, M.H. J. Biol. Chem. (1983) [Pubmed]
  5. Nucleotide sequence of the mannitol (mtl) operon in Escherichia coli. Davis, T., Yamada, M., Elgort, M., Saier, M.H. Mol. Microbiol. (1988) [Pubmed]
  6. Genetic analyses of the mannitol permease of Escherichia coli: isolation and characterization of a transport-deficient mutant which retains phosphorylation activity. Manayan, R., Tenn, G., Yee, H.B., Desai, J.D., Yamada, M., Saier, M.H. J. Bacteriol. (1988) [Pubmed]
  7. Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome. Lee, C.A., Saier, M.H. J. Bacteriol. (1983) [Pubmed]
  8. Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping. Lengeler, J. J. Bacteriol. (1975) [Pubmed]
  9. Insertion of the mannitol permease into the membrane of Escherichia coli. Possible involvement of an N-terminal amphiphilic sequence. Yamada, Y., Chang, Y.Y., Daniels, G.A., Wu, L.F., Tomich, J.M., Yamada, M., Saier, M.H. J. Biol. Chem. (1991) [Pubmed]
  10. Determination of a 17,484 bp nucleotide sequence around the 39 degrees region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs. Akagawa, E., Kurita, K., Sugawara, T., Nakamura, K., Kasahara, Y., Ogasawara, N., Yamane, K. Microbiology (Reading, Engl.) (1995) [Pubmed]
  11. Molecular analysis of the mannitol operon of Clostridium acetobutylicum encoding a phosphotransferase system and a putative PTS-modulated regulator. Behrens, S., Mitchell, W., Bahl, H. Microbiology (Reading, Engl.) (2001) [Pubmed]
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