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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

orf1  -  hypothetical protein

Escherichia coli

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Disease relevance of orf1


High impact information on orf1

  • Shotgun cloning experiments with restriction enzyme-digested genomic DNA from Morganella morganii 1, which expresses high levels of cephalosporinase, into the pBKCMV cloning vector gave a recombinant plasmid, pPON-1, which encoded four entire genes: ampC, ampR, an hybF family gene, and orf-1 of unknown function [6].
  • Through the use of transcriptional fusions, none of the putative tol (or tol-associated) genes were shown to be regulated by PhoPQ, bile, or the RcsC-RcsB two-component system; however, all of the genes (orfX, orf1, tolQRA) are predicted to be cotranscribed [7].
  • Two other genes (orf1 and orf6) conferring utilization of 4-hydroxybutyrate were identified during subcloning and sequencing of the inserts of pAH1 and pAH3 [8].
  • These ORFs were designated cpsIaA to cpsIaL, neu (neuraminic acid synthesis gene) A to D, orf1 and ung (uracil DNA glycosylase) [9].
  • DNA sequence analysis showed that there were two transcriptional terminators in the 3' end of this operon (downstream of orf1 and ung) [9].

Biological context of orf1


Associations of orf1 with chemical compounds

  • The lack of polymerization of the K40 polysaccharide indicates that orf1 encodes the K40 polymerase (Wzy) and that assembly of the K40 polysaccharide occurs via a Wzy-dependent pathway (in contrast to that of the O8 polysaccharide) [13].
  • A third gene (morC), exhibiting sequence identity to known reductases, and a fourth truncated gene encoding a putative glutamine reductase (orf1' ), were found downstream of morB [14].

Other interactions of orf1


Analytical, diagnostic and therapeutic context of orf1


  1. Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant. Troup, B., Jahn, M., Hungerer, C., Jahn, D. J. Bacteriol. (1994) [Pubmed]
  2. Biochemical and genetic characterization of 2-carboxybenzaldehyde dehydrogenase, an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7. Iwabuchi, T., Harayama, S. J. Bacteriol. (1997) [Pubmed]
  3. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains. Park, S.J., Ahn, W.S., Green, P.R., Lee, S.Y. Biotechnol. Bioeng. (2001) [Pubmed]
  4. Sequence analysis and characterization of sulfonamide resistance plasmid pRF-1 from Salmonella enterica serovar Choleraesuis. Haneda, T., Okada, N., Miki, T., Danbara, H. Plasmid (2004) [Pubmed]
  5. Characterization of the plasmid pMB1 from Bifidobacterium longum and its use for shuttle vector construction. Rossi, M., Brigidi, P., Gonzalez Vara y Rodriguez, A., Matteuzzi, D. Res. Microbiol. (1996) [Pubmed]
  6. Cloning, sequence analyses, expression, and distribution of ampC-ampR from Morganella morganii clinical isolates. Poirel, L., Guibert, M., Girlich, D., Naas, T., Nordmann, P. Antimicrob. Agents Chemother. (1999) [Pubmed]
  7. Salmonella enterica serovar typhimurium resistance to bile: identification and characterization of the tolQRA cluster. Prouty, A.M., Van Velkinburgh, J.C., Gunn, J.S. J. Bacteriol. (2002) [Pubmed]
  8. Construction of environmental DNA libraries in Escherichia coli and screening for the presence of genes conferring utilization of 4-hydroxybutyrate. Henne, A., Daniel, R., Schmitz, R.A., Gottschalk, G. Appl. Environ. Microbiol. (1999) [Pubmed]
  9. Molecular characterization of type-specific capsular polysaccharide biosynthesis genes of Streptococcus agalactiae type Ia. Yamamoto, S., Miyake, K., Koike, Y., Watanabe, M., Machida, Y., Ohta, M., Iijima, S. J. Bacteriol. (1999) [Pubmed]
  10. Use of chromosomal gene fusions to investigate the role of repetitive DNA in regulation of genes involved in lipopolysaccharide biosynthesis in Haemophilus influenzae. Szabo, M., Maskell, D., Butler, P., Love, J., Moxon, R. J. Bacteriol. (1992) [Pubmed]
  11. Streptococcal plasmid pIP501 has a functional oriT site. Wang, A., Macrina, F.L. J. Bacteriol. (1995) [Pubmed]
  12. Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli. Schäfer, A., Schwarzer, A., Kalinowski, J., Pühler, A. J. Bacteriol. (1994) [Pubmed]
  13. Assembly of the K40 antigen in Escherichia coli: identification of a novel enzyme responsible for addition of L-serine residues to the glycan backbone and its requirement for K40 polymerization. Amor, P.A., Yethon, J.A., Monteiro, M.A., Whitfield, C. J. Bacteriol. (1999) [Pubmed]
  14. Molecular cloning, nucleotide sequencing and expression of genes encoding a cytochrome P450 system involved in secondary amine utilization in Mycobacterium sp. strain RP1. Trigui, M., Pulvin, S., Truffaut, N., Thomas, D., Poupin, P. Res. Microbiol. (2004) [Pubmed]
  15. Regulation of the operon responsible for broad-spectrum mercury resistance in Streptomyces lividans 1326. Brünker, P., Rother, D., Sedlmeier, R., Klein, J., Mattes, R., Altenbuchner, J. Mol. Gen. Genet. (1996) [Pubmed]
  16. A 10 kb nucleotide sequence at the 5' flanking region (32 degrees) of srfAA of the Bacillus subtilis chromosome. Fujishima, Y., Yamane, K. Microbiology (Reading, Engl.) (1995) [Pubmed]
  17. Analysis of the genes responsible for the O-antigen synthesis in enterohaemorrhagic Escherichia coli O157. Shimizu, T., Yamasaki, S., Tsukamoto, T., Takeda, Y. Microb. Pathog. (1999) [Pubmed]
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