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Gene Review

dcuB  -  C4-dicarboxylate transporter, anaerobic;...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4116, JW4084, genF
 
 
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Disease relevance of dcuB

  • In addition, dcuB expression is strongly induced by C4-dicarboxylates, suggesting the presence of a novel C4-dicarboxylate-responsive regulator in E. coli [1].
 

High impact information on dcuB

  • Upon phosphorylation, DcuR bound specifically to dcuB promoter DNA [2].
  • A plasmid carrying the dcuSR operon restored the C4-dicarboxylate inducibility of dcuB expression in the dcuS mutant to levels exceeding those of the dcuS+ strain by approximately 1.8-fold [1].
  • The results show that dcuA is constitutively expressed whereas dcuB expression is highly regulated [3].
  • The expression of dcuB is entirely consistent with a primary role for DcuB in mediating C4-dicarboxylate transport during anaerobic fumarate respiration [3].
  • The dcuB gene is strongly activated anaerobically by FNR, repressed in the presence of nitrate by NarL, and subject to cyclic AMP receptor protein (CRP)-mediated catabolite repression [3].
 

Biological context of dcuB

  • In addition, dcuB is strongly induced by C4-dicarboxylates, suggesting that dcuB is under the control of an uncharacterized C4-dicarboxylate-responsive gene regulator [3].
  • The dcuA and dcuB genes encode related integral inner-membrane proteins, DcuA and DcuB (433 and 446 amino acid residues), which have 36% amino acid sequence identity [4].
  • The dcuA and dcuB genes of Escherichia coli encode homologous proteins that appear to function as independent and mutually redundant C4-dicarboxylate transporters during anaerobiosis [3].
  • The binding site of DcuR-P at the dcuB promoter was determined by DNase I footprinting [5].
 

Associations of dcuB with chemical compounds

  • Growth tests and transport studies with mutants containing insertionally inactivated chromosomal dcuA and dcuB genes show that their products perform analogous and mutually complementary roles as anaerobic dicarboxylate carriers [6].
  • In gel retardation assays with target promoters (frdA, dcuB, dctA), phosphoryl DcuR (DcuR-P) formed a high-affinity complex, with an apparent K(D) (app. K(D)) of 0.2-0.3 microM DcuR-P, and a low-affinity (app. K(D) 0.8-2 microM) complex [5].

References

 
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