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Gene Review:

glnA - glutamate--ammonia ligase

Synechocystis sp. PCC 6803

This record was discontinued.

Reyes, J.C. et al., Takatani, N. et al., Reyes, J.C. et al., Imamura, S. et al., García-Domínguez, M. et al., et al.

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Disease relevance of glnA

The altered steady-state expression levels of glnA, glnN, amt1, gifA and gifB in the PII-deficient mutant suggested that Synechocystis sp. strain PCC 6803 has a mechanism for regulation of the subset of the NtcA-regulated genes related directly to ammonium assimilation [1].
Our results demonstrate that NtcA plays a central role in GS regulation in cyanobacteria, stimulating transcription of the glnA gene (GS structural gene) and suppressing transcription of the GS inactivating factor genes gifA and gifB [2].

High impact information on glnA

We also confirmed the contribution of SigB and SigC to the transcription of other NtcA-dependent genes, glnA, sigE, and amt1, as in the case of glnB [3].
In the cyanobacterium Synechocystis sp. strain PCC 6803 we have previously reported the presence of two different proteins with glutamine synthetase activity: GSI, encoded by the glnA gene, and GSIII, encoded by the glnN gene [4].
Northern blot experiments and transcriptional fusions demonstrated that the glnA gene is highly transcribed in nitrate- or ammonium-grown cells and exhibits two- to fourfold-higher expression in nitrogen-starved cells [4].
Electron transport controls transcription of the glutamine synthetase gene (glnA) from the cyanobacterium Synechocystis sp. PCC 6803 [5].
Synthesis of the glnA transcript in the dark required the presence of glucose [5].

Chemical compound and disease context of glnA

A mutant lacking the glutamine synthetase gene (glnA) is impaired in the regulation of the nitrate assimilation system in the cyanobacterium Synechocystis sp. strain PCC 6803 [6].

Biological context of glnA

A dramatic decrease in the amount of glnA mRNA, a single transcript of about 1.6 kb, was observed after transfer to darkness, or after incubation with the electron transport inhibitors DCMU or DBMIB [5].

Analytical, diagnostic and therapeutic context of glnA

In addition, mobility shift assays demonstrated that purified, Escherichia coli-expressed Synechocystis NtcA protein binds to the promoter of the glnA gene [4].

References

Effects of PII deficiency on expression of the genes involved in ammonium utilization in the cyanobacterium Synechocystis sp. Strain PCC 6803. Takatani, N., Omata, T. Plant Cell Physiol. (2006) [Pubmed]
NtcA represses transcription of gifA and gifB, genes that encode inhibitors of glutamine synthetase type I from Synechocystis sp. PCC 6803. García-Domínguez, M., Reyes, J.C., Florencio, F.J. Mol. Microbiol. (2000) [Pubmed]
Growth phase-dependent activation of nitrogen-related genes by a control network of group 1 and group 2 sigma factors in a cyanobacterium. Imamura, S., Tanaka, K., Shirai, M., Asayama, M. J. Biol. Chem. (2006) [Pubmed]
Transcription of glutamine synthetase genes (glnA and glnN) from the cyanobacterium Synechocystis sp. strain PCC 6803 is differently regulated in response to nitrogen availability. Reyes, J.C., Muro-Pastor, M.I., Florencio, F.J. J. Bacteriol. (1997) [Pubmed]
Electron transport controls transcription of the glutamine synthetase gene (glnA) from the cyanobacterium Synechocystis sp. PCC 6803. Reyes, J.C., Florencio, F.J. Plant Mol. Biol. (1995) [Pubmed]
A mutant lacking the glutamine synthetase gene (glnA) is impaired in the regulation of the nitrate assimilation system in the cyanobacterium Synechocystis sp. strain PCC 6803. Reyes, J.C., Florencio, F.J. J. Bacteriol. (1994) [Pubmed]

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