Disease relevance of polymerase

• Tomato spotted wilt virus L RNA encodes a putative RNA polymerase [1].
• The insert contained in plasmid pTSW1 was repaired and amplified by polymerase chain reaction, and the complete nucleocapsid protein gene was introduced into Nicotiana tabacum 'Samsun' by leaf disk transformation using Agrobacterium tumefaciens [2].
• The enlargement is based on an insertion of 365 nts in the IGR that may have resulted from stuttering of the viral polymerase or non-homologous recombination [3].
• A genetic tree comparing the available polymerase proteins of the Bunyaviridae provides insights into the phylogenetic relationships within this large family [4].
• Inspection of the sequences in the primers on nairovirus, bunyavirus and phlebovirus mRNAs suggests that in some cases polymerase slippage occurs shortly after initiation, resulting in a partial reiteration of the 5'-terminal nucleotides of the viral RNA [5].

High impact information on polymerase

• Using reverse transcriptase in conjunction with PCR amplification, we have obtained the nucleotide sequence of the L RNA segment, which encodes the viral polymerase in a single large open reading frame [4].
• Comparison of the amino acid sequence of the LAC L protein with the sequence of other polymerases from members of the Bunyaviridae, demonstrated the presence of several conserved motifs, some of which are characteristic of many polymerase proteins [4].

References