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nucleocapsid protein  -  nucleocapsid protein

Tomato spotted wilt virus

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Disease relevance of nucleocapsid protein


High impact information on nucleocapsid protein


Biological context of nucleocapsid protein

  • The sequence contains two open reading frames (ORFs), one in the viral sense which encodes a protein with a predicted Mr of 52.4K and one in the viral complementary sense which encodes the viral nucleocapsid protein of Mr 28.8K [8].
  • In vitro gene expression strategy was used for the production of polyclonal antiserum to the nucleocapsid protein (NP) of Groundnut bud necrosis virus (GBNV) [9].
  • A recombinant plasmid containing the entire tomato spotted with virus (TSWV) nucleocapsid gene, with the exception of nucleotide encoding three N-terminal amino acids, was isolated by screening a complementary DNA library, prepared against random primed viral RNA, using a specific monoclonal antibody [4].
  • The GBNV NP gene from cowpea isolate was cloned into 6x His-tagged UA cloning vector and expressed in Escherichia coli [M15] cells [9].
  • In sensitised emission experiments, energy transfer was observed to take place from CFP to YFP when the two fluorophores were fused to TSWV N protein, indicating strongly homotypic interaction of the N proteins [10].

Anatomical context of nucleocapsid protein

  • Furthermore, immunogold labeling of tissue sections of TSWV-infected N. rustica plants showed that this protein was found associated with nucleocapsid aggregates in the cytoplasm and in close association with plasmodesmata [11].
  • The accumulation of large amounts of N and NSs protein, the occurrence of several vesicles with virus particles in the salivary glands and the massive numbers of virus particles in the salivary gland ducts demonstrate that the salivary glands are a major site of TSWV replication [12].

Analytical, diagnostic and therapeutic context of nucleocapsid protein

  • Using polyclonal antibodies against the viral nucleocapsid protein (N) and indirect immunofluorescent staining, discrete spots with strong signals were observed in the cytoplasm at 48 h post-inoculation in the cell cultures of a F. occidentalis, and a T. tabaci population which failed to transmit the virus [13].
  • Immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus [9].
  • Twenty tomato spotted wilt virus (TSWV) isolates were serologically compared in ELISA employing five different procedures using a rabbit polyclonal antiserum against nucleocapsid proteins (NuAbR) and mouse monoclonal antibodies (MAbs), two directed to nucleocapsid proteins (N1 and N2) and four directed to glycoproteins G1 to G4 [14].
  • Full-length N, N amino and carboxy halves, and two N carboxy-terminal regions were expressed and isolated by metal chelate affinity chromatography [15].
  • Average values between 1300 and 2200 nm of nucleocapsid lengths could be related to dimensions estimated by electron microscopy, thereby validating a filamentous configuration of the TSWV ribonucleoproteins [16].


  1. Homotypic interaction and multimerization of nucleocapsid protein of tomato spotted wilt tospovirus: identification and characterization of two interacting domains. Uhrig, J.F., Soellick, T.R., Minke, C.J., Philipp, C., Kellmann, J.W., Schreier, P.H. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  2. Hantavirus N protein exhibits genus-specific recognition of the viral RNA panhandle. Mir, M.A., Brown, B., Hjelle, B., Duran, W.A., Panganiban, A.T. J. Virol. (2006) [Pubmed]
  3. Expression and characterization of a soluble form of tomato spotted wilt virus glycoprotein GN. Whitfield, A.E., Ullman, D.E., German, T.L. J. Virol. (2004) [Pubmed]
  4. Resistance to tomato spotted wilt virus infection in transgenic tobacco expressing the viral nucleocapsid gene. MacKenzie, D.J., Ellis, P.J. Mol. Plant Microbe Interact. (1992) [Pubmed]
  5. Tomato spotted wilt virus Gc and N proteins interact in vivo. Snippe, M., Willem Borst, J., Goldbach, R., Kormelink, R. Virology (2007) [Pubmed]
  6. Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants. Pang, S.Z., Jan, F.J., Gonsalves, D. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  7. A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance. Jan, F.J., Fagoaga, C., Pang, S.Z., Gonsalves, D. J. Gen. Virol. (2000) [Pubmed]
  8. The S RNA segment of tomato spotted wilt virus has an ambisense character. de Haan, P., Wagemakers, L., Peters, D., Goldbach, R. J. Gen. Virol. (1990) [Pubmed]
  9. Immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus. Jain, R.K., Pandey, A.N., Krishnareddy, M., Mandal, B. J. Virol. Methods (2005) [Pubmed]
  10. The use of fluorescence microscopy to visualise homotypic interactions of tomato spotted wilt virus nucleocapsid protein in living cells. Snippe, M., Borst, J.W., Goldbach, R., Kormelink, R. J. Virol. Methods (2005) [Pubmed]
  11. Expression and subcellular location of the NSM protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. Kormelink, R., Storms, M., Van Lent, J., Peters, D., Goldbach, R. Virology (1994) [Pubmed]
  12. Multiplication of tomato spotted wilt virus in its insect vector, Frankliniella occidentalis. Wijkamp, I., van Lent, J., Kormelink, R., Goldbach, R., Peters, D. J. Gen. Virol. (1993) [Pubmed]
  13. Multiplication of tomato spotted wilt virus in primary cell cultures derived from two thrips species. Nagata, T., Storms, M.M., Goldbach, R., Peters, D. Virus Res. (1997) [Pubmed]
  14. Serological differentiation of 20 isolates of tomato spotted wilt virus. de Avila, A.C., Huguenot, C., Resende, R.d.e. .O., Kitajima, E.W., Goldbach, R.W., Peters, D. J. Gen. Virol. (1990) [Pubmed]
  15. Characterization of the nucleic acid binding properties of tomato spotted wilt virus nucleocapsid protein. Richmond, K.E., Chenault, K., Sherwood, J.L., German, T.L. Virology (1998) [Pubmed]
  16. Visual representation by atomic force microscopy (AFM) of tomato spotted wilt virus ribonucleoproteins. Kellmann, J.W., Liebisch, P., Schmitz, K.P., Piechulla, B. Biol. Chem. (2001) [Pubmed]
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