Disease relevance of fliC

• The E. coli detection assay consists of a triplex PCR amplification targeting genes that encode 16S ribosomal RNA, the fliC flagellar antigen, and the sltI shigatoxin [1].
• Cloning of the metal-regulated gene, hybridization to the ordered phage lambda bank of the E. coli chromosome, and sequencing of DNA adjacent to the luxAB fusion revealed that the insertion occurred within the fliC (hag) gene of E. coli [2].
• The fllA genes are highly homologous to the E. coli fliC gene but distantly related to the Salmonella fljB gene [3].

High impact information on fliC

• Two genotypes of the fliC gene encoding H8 (the fliC-H8 gene) were identified [4].
• Fifty-seven Escherichia coli O26 strains isolated from patients in six countries were investigated by PCR restriction fragment length polymorphism (RFLP) analysis of the flagellin-encoding (fliC) gene (fliC RFLP analysis) [5].
• The three strains with the H32 fliC RFLP pattern belonged to serotype O26:H32, and all were eae negative [5].
• The fliC genes of 14 selected E. coli O26:H11 strains isolated between 1964 and 1999 had identical nucleotide sequences [5].
• The possible significance of multiple fliC in swarmer cell differentiation is discussed [6].

Biological context of fliC

• Within these fliC genotypes, a high degree of homogeneity for the presence of disease-associated genes was found [7].
• The fliC RFLP analysis revealed only two different patterns among the 57 strains [5].
• The second and third ORFs are highly homologous to each other and to fliC genes from many other Gram- bacteria [6].

Analytical, diagnostic and therapeutic context of fliC

• The fliC genotyping method is advantageous over serotyping and is useful for epidemiological investigations and studies of the evolution of STEC clones [4].
• Sequence analysis of the flagellin gene, fliC, reveals a central variable region and the highly conserved N- and C-termini [8].
• The proposed determination of fliC restriction patterns should be helpful for epidemiological studies [9].
• The structure of the fliC gene and its surrounding region was shown to be the same in both phase variants, but Northern blot analysis revealed that fliC and fliD were, respectively, not and weakly transcribed in phase II variants [10].
• E. coli O157:H7 isolates were fingerprinted by pulsed-field gel electrophoresis and subjected to multiplex PCR procedures for amplification of E. coli O157:H7 genes stx1, stx2, eaeA, fliC, rfbEO157, and hlyA [11].

References