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Gene Review

nodS  -  nodulation protein NodS

Sinorhizobium fredii NGR234

 
 
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Disease relevance of nodS

  • Likewise, nodS- and nodU-like genes were identified in Rhizobium sp. strain NGR234 (A. Lewin, E. Cervantes, W. Chee-Hoong, and W. J. Broughton, Molecular Plant-Microbe Interactions 3:317-326, 1990) in which nodS confers host specificity for Leucaena leucocephala.(ABSTRACT TRUNCATED AT 250 WORDS)[1]
  • Identification of nodS and nodU, two inducible genes inserted between the Bradyrhizobium japonicum nodYABC and nodIJ genes [1].
  • Identification of nodSUIJ genes in Nod locus 1 of Azorhizobium caulinodans: evidence that nodS encodes a methyltransferase involved in Nod factor modification [2].
  • The A. caulinodans nodS gene was expressed in Escherichia coli and a glutathione-S-transferase-NodS fusion protein purified [3].
 

High impact information on nodS

  • Involvement of nodS in N-methylation and nodU in 6-O-carbamoylation of Rhizobium sp. NGR234 nod factors [4].
  • The NodBCS metabolites were partially converted to lipo-chitooligosaccharides when the nodABCS genes were expressed, showing that NodA was involved in the acylation and acted after NodS [5].
  • Several lines of evidence indicate that the nodS gene located in the nodABCSUIJ operon is implicated in the methylation of Nod factors [3].
  • NodS is an S-adenosyl-L-methionine-dependent methyltransferase that methylates chitooligosaccharides deacetylated at the non-reducing end [3].
  • Here, we present an in vitro assay showing that NodS from either A. caulinodans or Rhizobium species NGR234 methylates end-deacetylated chitooligosaccharides, using [3H-methyl]-SAM as a methyl donor [3].
 

Chemical compound and disease context of nodS

 

Biological context of nodS

  • Although both nucleotide and deduced amino acid sequences of the reading frames are homologous between the two strains, there are gaps within the promoter region and the 5'-end of nodS of USDA257 [6].
  • Complementation experiments with a series of subclones established that nodS and its associated nod-box promoter from NGR234 are sufficient to confer this extended host-range phenotype to L. leucocephala [6].
  • Nucleotide sequence of the Rhizobium etli nodS gene [7].
  • Surprisingly, in transconjugant strains of Mesorhizobium loti, Rhizobium etli, and Rhizobium tropici carrying nodL, such acetylation of LCOs prevents the endogenous nodS-dependent transfer of the N-methyl group that is found as a substituent of the acylated nitrogen atom [8].
 

Associations of nodS with chemical compounds

  • Using translational fusions of lacZ to nodC, nodS, and nodU, the expression of these genes was shown to be inducible by the isoflavone daidzein and depended on transcription from a DNA region farther upstream [1].
  • A naringenin-inducible nodS-dependent protein of approximately 25 kDa could be cross-linked to radiolabelled SAM [2].

References

  1. Identification of nodS and nodU, two inducible genes inserted between the Bradyrhizobium japonicum nodYABC and nodIJ genes. Göttfert, M., Hitz, S., Hennecke, H. Mol. Plant Microbe Interact. (1990) [Pubmed]
  2. Identification of nodSUIJ genes in Nod locus 1 of Azorhizobium caulinodans: evidence that nodS encodes a methyltransferase involved in Nod factor modification. Geelen, D., Mergaert, P., Geremia, R.A., Goormachtig, S., Van Montagu, M., Holsters, M. Mol. Microbiol. (1993) [Pubmed]
  3. NodS is an S-adenosyl-L-methionine-dependent methyltransferase that methylates chitooligosaccharides deacetylated at the non-reducing end. Geelen, D., Leyman, B., Mergaert, P., Klarskov, K., Van Montagu, M., Geremia, R., Holsters, M. Mol. Microbiol. (1995) [Pubmed]
  4. Involvement of nodS in N-methylation and nodU in 6-O-carbamoylation of Rhizobium sp. NGR234 nod factors. Jabbouri, S., Fellay, R., Talmont, F., Kamalaprija, P., Burger, U., Relić, B., Promé, J.C., Broughton, W.J. J. Biol. Chem. (1995) [Pubmed]
  5. Biosynthesis of Azorhizobium caulinodans Nod factors. Study of the activity of the NodABCS proteins by expression of the genes in Escherichia coli. Mergaert, P., D'Haeze, W., Geelen, D., Promé, D., Van Montagu, M., Geremia, R., Promé, J.C., Holsters, M. J. Biol. Chem. (1995) [Pubmed]
  6. Differential expression of nodS accounts for the varied abilities of Rhizobium fredii USDA257 and Rhizobium sp. strain NGR234 to nodulate Leucaena spp. Krishnan, H.B., Lewin, A., Fellay, R., Broughton, W.J., Pueppke, S.G. Mol. Microbiol. (1992) [Pubmed]
  7. Nucleotide sequence of the Rhizobium etli nodS gene. Villalobos, M.A., Nava, N., Vázquez, M., Quinto, C. Gene (1994) [Pubmed]
  8. Rhizobial NodL O-acetyl transferase and NodS N-methyl transferase functionally interfere in production of modified Nod factors. López-Lara, I.M., Kafetzopoulos, D., Spaink, H.P., Thomas-Oates, J.E. J. Bacteriol. (2001) [Pubmed]
 
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