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MeSH Review

Protein Footprinting

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High impact information on Protein Footprinting


Biological context of Protein Footprinting


Associations of Protein Footprinting with chemical compounds


Gene context of Protein Footprinting

  • In this work, we employed a mass spectrometric protein footprinting method of single amino acid resolution to investigate the interactions of the entire heterotrimeric hRPA with ssDNA [12].
  • Protein footprinting data suggest that Rev interacts specifically with amino acids within the 196-208 region of p32 [13].
  • A series of N-terminal, C-terminal, and internal deletion mutants, as well as protein footprinting, were used to identify the target regions in GAD65 [14].
  • Protein footprinting analysis, whereby polypeptide fragments generated on protease treatment of immune complexes are studied, indicated considerable heterogeneity in antibody recognition of IA-2, even between sera with similar reactivity to deletion mutants [15].
  • Mapping the G-actin binding surface of cofilin using synchrotron protein footprinting [16].


  1. Amino-terminal sequences of sigmaN (sigma54) inhibit RNA polymerase isomerization. Cannon, W., Gallegos, M.T., Casaz, P., Buck, M. Genes Dev. (1999) [Pubmed]
  2. RNA-protein interactions of an archaeal homotetrameric splicing endoribonuclease with an exceptional evolutionary history. Lykke-Andersen, J., Garrett, R.A. EMBO J. (1997) [Pubmed]
  3. Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a specific trans-acting factor. Lefevre, C., Imagawa, M., Dana, S., Grindlay, J., Bodner, M., Karin, M. EMBO J. (1987) [Pubmed]
  4. Protein footprinting at cysteines: probing ATP-modulated contacts in cysteine-substitution mutants of yeast DNA topoisomerase II. Tu, B.P., Wang, J.C. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  5. Determinants of RNA polymerase alpha subunit for interaction with beta, beta', and sigma subunits: hydroxyl-radical protein footprinting. Heyduk, T., Heyduk, E., Severinov, K., Tang, H., Ebright, R.H. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  6. Mapping interactions of Escherichia coli GreB with RNA polymerase and ternary elongation complexes. Loizos, N., Darst, S.A. J. Biol. Chem. (1999) [Pubmed]
  7. The upstream region of the human gamma-globin gene promoter. Identification and functional analysis of nuclear protein binding sites. McDonagh, K.T., Lin, H.J., Lowrey, C.H., Bodine, D.M., Nienhuis, A.W. J. Biol. Chem. (1991) [Pubmed]
  8. Cellular or viral protein binding to a cytomegalovirus promoter transcription initiation site: effects on transcription. Macias, M.P., Huang, L., Lashmit, P.E., Stinski, M.F. J. Virol. (1996) [Pubmed]
  9. Exploring protein interfaces with a general photochemical reagent. Gómez, G.E., Cauerhff, A., Craig, P.O., Goldbaum, F.A., Delfino, J.M. Protein Sci. (2006) [Pubmed]
  10. Protein footprinting by the combined use of reversible and irreversible lysine modifications. Hanai, R., Wang, J.C. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  11. Fluorescence-based protein footprinting using histidine-tagged protein. Rajendrakumar, G.V., Adhya, S. BioTechniques (1998) [Pubmed]
  12. Mass spectrometric identification of lysines involved in the interaction of human replication protein a with single-stranded DNA. Shell, S.M., Hess, S., Kvaratskhelia, M., Zou, Y. Biochemistry (2005) [Pubmed]
  13. In vitro interaction between human immunodeficiency virus type 1 Rev protein and splicing factor ASF/SF2-associated protein, p32. Tange, T.O., Jensen, T.H., Kjems, J. J. Biol. Chem. (1996) [Pubmed]
  14. Autoreactive epitopes defined by diabetes-associated human monoclonal antibodies are localized in the middle and C-terminal domains of the smaller form of glutamate decarboxylase. Richter, W., Shi, Y., Baekkeskov, S. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  15. Cross reactivity between IA-2 and phogrin/IA-2beta in binding of autoantibodies in IDDM. Hatfield, E.C., Hawkes, C.J., Payton, M.A., Christie, M.R. Diabetologia (1997) [Pubmed]
  16. Mapping the G-actin binding surface of cofilin using synchrotron protein footprinting. Guan, J.Q., Vorobiev, S., Almo, S.C., Chance, M.R. Biochemistry (2002) [Pubmed]
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