The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

LSP1 regulates anti-IgM induced apoptosis in WEHI-231 cells and normal immature B-cells.

Expression of LSP1, a 330 amino acid intracellular phosphoprotein, is restricted to lymphocytes, macrophages and neutrophils. In B-lymphoma cell lines LSP1 co-caps with membrane IgM after stimulation with anti-IgM. We used the LSP1+ B-lymphoma cell line WEHI-231/89 and normal lipopolysaccharide treated immature B-cells from Lsp1-/- and wild type mice to determine a role for LSP1 in signaling through membrane IgM. WEHI-231/89 cells were transfected with a truncated LSP1 protein containing the COOH-terminal residues 179-330. The three transfectants expressing the LSP1 truncate were significantly more susceptible to anti-IgM induced apoptosis than the parental cells or G418r control cell lines, while anti-IgM induced growth arrest was not affected. Expression of the LSP1 truncate increased the extent of anti-IgM induced loss of mitochondrial membrane potential, delta(psi)m indicating that LSP1 acts at a early stage in BCR mediated apoptosis. Expression of the LSP1 truncate in WEHI-231/89 cells increased susceptibility to ionomycin induced apoptosis but had no effect on apoptosis induced by nocodazole, sorbitol, C2-ceramide or H2O2. A role for LSP1 in anti-IgM induced apoptosis was confirmed using normal immature B-cells from 129/SvJ-Lsp1-/- mice which were less susceptible to anti-IgM induced apoptosis than those isolated from wild-type 129/SvJ mice. These results suggest that LSP1 regulates a Ca2+-dependent step in the induction phase of anti-IgM mediated apoptosis.[1]

References

  1. LSP1 regulates anti-IgM induced apoptosis in WEHI-231 cells and normal immature B-cells. Jongstra-Bilen, J., Wielowieyski, A., Misener, V., Jongstra, J. Mol. Immunol. (1999) [Pubmed]
 
WikiGenes - Universities