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Gene Review

Lsp1  -  lymphocyte specific 1

Mus musculus

Synonyms: 52 kDa phosphoprotein, Lsp-1, Lymphocyte-specific antigen WP34, Lymphocyte-specific protein 1, Pp52, ...
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Disease relevance of Lsp1

  • This study examines LSP1-deficient (Lsp1(-/-)) mice for the development of myeloid and lymphocytic cell populations and their response to the development of peritonitis induced by thioglycollate (TG) and to a T-dependent antigen [1].
  • When the putative coding region of p50 cDNA was expressed in E. coli, the product showed an apparent molecular mass of 50 kDa on SDS-PAGE [2].
  • A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA [3].
  • SAP (SLAM-associated protein) is a small lymphocyte-specific signalling molecule that is defective or absent in patients with X-linked lymphoproliferative syndrome (XLP) [4].
  • The MPC11 LTRs were five- to sixfold more active than an LS cDNA LTR when tested for promoter activity by transfection into plasmacytoma cells [5].

High impact information on Lsp1


Chemical compound and disease context of Lsp1

  • Studies with three independent sets of well-characterized parental CD45+, mutant CD45-, and revertant CD45+ lymphoma cell lines indicate that loss of CD45 increases the phosphorylation of the src-related leukocyte-specific tyrosine protein kinase p56lck on tyrosine-505, a putative negative regulatory site [11].
  • Here we report that approximately 10% of the total intracellular LSP1 protein is associated with the Nonidet P-40 insoluble cytoskeleton of the mIgM+, mIgD+ B lymphoma cell line BAL17 [12].
  • Here we develop a novel high-throughput assay by allowing a chemical library to compete with phage display peptide binding and identify a novel small-molecule ligand to the leukocyte-specific alpha(M)beta(2) integrin [13].
  • Earlier studies from this laboratory suggested that higher homologues in a series could have greater affinity for system L. A higher homologue of BCH, 2-aminobicyclo[3.2.1]octane-2-carboxylic acid (ABOCA), has been synthesized and studied as a substrate and competitor for amino acid transport systems of the sarcoma 37 (S37) ascites cell [14].
  • Preabsorption of the cell extract prior to immunoprecipitation with an excess of formalin-fixed Staphylococcus aureus, complexed with preimmune normal rabbit serum IgG, specifically removed the kinase phosphorylating p50 [15].

Biological context of Lsp1


Anatomical context of Lsp1

  • The level of peritoneal lymphocytes is decreased in Lsp1(-/-) mice without affecting a particular lymphocytic subset [1].
  • Lsp1(-/-) mice exhibit significantly higher levels of resident macrophages in the peritoneum compared to wild-type (wt) mice, whereas the development of myeloid cells is normal [1].
  • Using LSP1-deficient (Lsp1(-/-)) mice, we show increased neutrophil migration into mouse knee joints during zymosan-induced acute inflammation, an inflammatory model in which the number of resident synoviocytes are not affected by LSP1-deficiency [17].
  • We used the LSP1+ B-lymphoma cell line WEHI-231/89 and normal lipopolysaccharide treated immature B-cells from Lsp1-/- and wild type mice to determine a role for LSP1 in signaling through membrane IgM [20].
  • A new lymphocyte-specific gene which encodes a putative Ca2+-binding protein is not expressed in transformed T lymphocyte lines [21].

Associations of Lsp1 with chemical compounds


Physical interactions of Lsp1


Regulatory relationships of Lsp1


Other interactions of Lsp1


Analytical, diagnostic and therapeutic context of Lsp1


  1. LSP1 modulates leukocyte populations in resting and inflamed peritoneum. Jongstra-Bilen, J., Misener, V.L., Wang, C., Ginzberg, H., Auerbach, A., Joyner, A.L., Downey, G.P., Jongstra, J. Blood (2000) [Pubmed]
  2. Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes. Matsumoto, N., Kojima, S., Osawa, T., Toyoshima, S. J. Biochem. (1995) [Pubmed]
  3. A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA. Marth, J.D., Peet, R., Krebs, E.G., Perlmutter, R.M. Cell (1985) [Pubmed]
  4. SAP couples Fyn to SLAM immune receptors. Chan, B., Lanyi, A., Song, H.K., Griesbach, J., Simarro-Grande, M., Poy, F., Howie, D., Sumegi, J., Terhorst, C., Eck, M.J. Nat. Cell Biol. (2003) [Pubmed]
  5. Selective expression of intracisternal A-particle genes in established mouse plasmacytomas. Lueders, K.K., Fewell, J.W., Morozov, V.E., Kuff, E.L. Mol. Cell. Biol. (1993) [Pubmed]
  6. Association of transcriptionally silent genes with Ikaros complexes at centromeric heterochromatin. Brown, K.E., Guest, S.S., Smale, S.T., Hahm, K., Merkenschlager, M., Fisher, A.G. Cell (1997) [Pubmed]
  7. Differential requirements for alpha4 integrins during fetal and adult hematopoiesis. Arroyo, A.G., Yang, J.T., Rayburn, H., Hynes, R.O. Cell (1996) [Pubmed]
  8. Interaction of the unique N-terminal region of tyrosine kinase p56lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs. Turner, J.M., Brodsky, M.H., Irving, B.A., Levin, S.D., Perlmutter, R.M., Littman, D.R. Cell (1990) [Pubmed]
  9. The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck. Veillette, A., Bookman, M.A., Horak, E.M., Bolen, J.B. Cell (1988) [Pubmed]
  10. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements. Grosschedl, R., Baltimore, D. Cell (1985) [Pubmed]
  11. Expression of CD45 alters phosphorylation of the lck-encoded tyrosine protein kinase in murine lymphoma T-cell lines. Ostergaard, H.L., Shackelford, D.A., Hurley, T.R., Johnson, P., Hyman, R., Sefton, B.M., Trowbridge, I.S. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  12. The lymphocyte-specific protein LSP1 is associated with the cytoskeleton and co-caps with membrane IgM. Klein, D.P., Galea, S., Jongstra, J. J. Immunol. (1990) [Pubmed]
  13. Stabilization of the activated alphaMbeta2 integrin by a small molecule inhibits leukocyte migration and recruitment. Björklund, M., Aitio, O., Stefanidakis, M., Suojanen, J., Salo, T., Sorsa, T., Koivunen, E. Biochemistry (2006) [Pubmed]
  14. Interaction of 2-aminobicyclo[3.2.1]octane-2-carboxylic acid with the amino acid transport systems of the sarcoma 37 murine ascites tumor cell. Elliott, M.S., Matthews, R.H., Minton, J.P., Zand, R. Biochemistry (1981) [Pubmed]
  15. Serine kinase activity associated with Maloney murine sarcoma virus-124-encoded p37mos. Maxwell, S.A., Arlinghaus, R.B. Virology (1985) [Pubmed]
  16. LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration. Liu, L., Cara, D.C., Kaur, J., Raharjo, E., Mullaly, S.C., Jongstra-Bilen, J., Jongstra, J., Kubes, P. J. Exp. Med. (2005) [Pubmed]
  17. Modulation of Mac-1 (CD11b/CD18)-mediated adhesion by the leukocyte-specific protein 1 is key to its role in neutrophil polarization and chemotaxis. Wang, C., Hayashi, H., Harrison, R., Chiu, B., Chan, J.R., Ostergaard, H.L., Inman, R.D., Jongstra, J., Cybulsky, M.I., Jongstra-Bilen, J. J. Immunol. (2002) [Pubmed]
  18. Expression of mouse LSP1/S37 isoforms. S37 is expressed in embryonic mesenchymal cells. Misener, V.L., Hui, C., Malapitan, I.A., Ittel, M.E., Joyner, A.L., Jongstra, J. J. Cell. Sci. (1994) [Pubmed]
  19. The mouse Lsp1 and Tnnt3 genes are 4.3 kb apart on distal mouse chromosome 7. Misener, V.L., Wielowieyski, A., Brennan, L.A., Beebakhee, G., Jongstra, J. Mamm. Genome (1998) [Pubmed]
  20. LSP1 regulates anti-IgM induced apoptosis in WEHI-231 cells and normal immature B-cells. Jongstra-Bilen, J., Wielowieyski, A., Misener, V., Jongstra, J. Mol. Immunol. (1999) [Pubmed]
  21. A new lymphocyte-specific gene which encodes a putative Ca2+-binding protein is not expressed in transformed T lymphocyte lines. Jongstra, J., Tidmarsh, G.F., Jongstra-Bilen, J., Davis, M.M. J. Immunol. (1988) [Pubmed]
  22. Characterization of the 50 kDa protein phosphorylated in concanavalin A-stimulated mouse T cells. Matsumoto, N., Toyoshima, S., Osawa, T. J. Biochem. (1993) [Pubmed]
  23. Low expression of the Lsp1 gene in early mouse T-lymphomas induced by N-methyl-N-nitrosourea. Brennan, L., Jongstra, J. Carcinogenesis (1996) [Pubmed]
  24. B lymphocyte-specific protein binding near an immunoglobulin kappa-chain gene J segment. Weaver, D., Baltimore, D. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  25. A dominant-negative transgene defines a role for p56lck in thymopoiesis. Levin, S.D., Anderson, S.J., Forbush, K.A., Perlmutter, R.M. EMBO J. (1993) [Pubmed]
  26. Increased T-cell apoptosis and terminal B-cell differentiation induced by inactivation of the Ets-1 proto-oncogene. Bories, J.C., Willerford, D.M., Grévin, D., Davidson, L., Camus, A., Martin, P., Stéhelin, D., Alt, F.W. Nature (1995) [Pubmed]
  27. The adaptor protein Gads (Grb2-related adaptor downstream of Shc) is implicated in coupling hemopoietic progenitor kinase-1 to the activated TCR. Liu, S.K., Smith, C.A., Arnold, R., Kiefer, F., McGlade, C.J. J. Immunol. (2000) [Pubmed]
  28. Leukocyte-specific gene 1 protein (LSP1) is involved in chemokine KC-activated cytoskeletal reorganization in murine neutrophils in vitro. Hannigan, M., Zhan, L., Ai, Y., Huang, C.K. J. Leukoc. Biol. (2001) [Pubmed]
  29. Lineage-specific regulation of the murine RAG-2 promoter: GATA-3 in T cells and Pax-5 in B cells. Kishi, H., Wei, X.C., Jin, Z.X., Fujishiro, Y., Nagata, T., Matsuda, T., Muraguchi, A. Blood (2000) [Pubmed]
  30. The B lymphocyte-specific coactivator BOB.1/OBF.1 is required at multiple stages of B-cell development. Hess, J., Nielsen, P.J., Fischer, K.D., Bujard, H., Wirth, T. Mol. Cell. Biol. (2001) [Pubmed]
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