Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Hannon, K. et al.

Temporal and quantitative analysis of myogenic regulatory and growth factor gene expression in the developing mouse embryo.

Using a reverse transcription/polymerase chain reaction method, the temporal pattern of expression of the myogenic regulatory genes (myf5, myogenin, MRF4, myo D) was quantitated in developing mouse muscle (whole embryo: 6.5 to 12.5 days postcoitum (dpc); front limb buds: 9.5 to 12.5 dpc; hind limb buds: 11.5 to 14.5 dpc) and related to expression of TGF-beta 1, b-FGF, IGF-I, and IGF-II. Myf5 was the first myogenic regulatory factor to appear in both the whole embryo and front limb bud, with expression evident 7.5 and 9.5 dpc, respectively. A transient peak of MRF4 expression occurred 10.5 dpc in both the whole embryo and the front limb bud. Myogenin and myo D expression in the whole embryo was detected 8.5 and 9.5 dpc, respectively. In the front limb bud myogenin and myo D expression was not detected until 10.5 dpc. In the hind limb bud myf5, myogenin, and MRF4 expression was detected 11.5 dpc. Myo D expression was not detected until 12.5 dpc. With respect to growth factor expression, in the front limb bud TGF-beta 1, IGF-I, and IGF-II were evident 9.5 dpc, while bFGF was not detected until 10.5 dpc. In the hind limb bud TGF-beta 1, bFGF, IGF-I, and IGF-II expression was detected 11.5 dpc. These results show that in both the whole embryo and limb buds, all four myogenic regulatory factors are involved in the initiation of the myogenic program. We also show myf5 expression in the 9.5-dpc front limb bud, suggesting its expression in the somite-derived migrating muscle precursor cells. Correlations between growth factor-mediated myoblast proliferation and myogenic differentiation are discussed.[1]

References

Temporal and quantitative analysis of myogenic regulatory and growth factor gene expression in the developing mouse embryo. Hannon, K., Smith, C.K., Bales, K.R., Santerre, R.F. Dev. Biol. (1992) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.