Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete.
A novel, Gram-negative, non-motile, non-sporulating, rod-shaped bacterium isolated from a viscous two-phase olive-oil mill waste ('alpeorujo') is described. The strain, designated AW-6(T), is an obligate aerobe, forming irregular, pigmented creamy white colonies. The pH and temperature ranges for growth were pH 5-8 and 5-45 degrees C, with optimal pH and temperature for growth of pH 6-7 and 28-32 degrees C, respectively. Strain AW-6(T) was chemo-organotrophic and utilized mostly d(+)-glucose, protocatechuate and d(+)-xylose, followed by l-cysteine, d(-)-fructose, d(+)-galactose, l-histidine, lactose, sorbitol and sucrose. Menaquinone-7 was detected in the respiratory chain of strain AW-6(T). The major fatty acids of strain AW-6(T) were C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, iso-C(15 : 0), iso-C(17 : 0) 3-OH and C(16 : 0). The closest phylogenetic relative of strain AW-6(T) was clone BIti35 (89.7 % 16S rRNA gene sequence similarity), while Sphingobacterium thalpophilum DSM 11723(T) was the closest recognized relative within the Sphingobacteriaceae (88.2 % similarity). Strain AW-6(T) showed a low level of DNA-DNA relatedness to S. thalpophilum DSM 11723(T) (33.8-37.0 %). The DNA G+C content of strain AW-6(T) was 45.6 mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain AW-6(T) from members of the genera Sphingobacterium and Pedobacter. Thus, strain AW-6(T) is considered to represent a novel species of a new genus within the family Sphingobacteriaceae, for which the name Olivibacter sitiensis gen. nov., sp. nov. is proposed. The type strain is AW-6(T) (=DSM 17696(T)=CECT 7133(T)).[1]References
- Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete. Ntougias, S., Fasseas, C., Zervakis, G.I. Int. J. Syst. Evol. Microbiol. (2007) [Pubmed]
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